渔业研究2016，Vol.38Issue(6)：460-465,6.DOI:10.14012/j.cnki.fjsc.2016.06.005
免疫荧光层析快速检测河豚毒素的研究
The study of immunofluorescence chromatography detection in tetrodotoxin
摘要
Abstract
In the southeast coastal area of China, tetrodotoxin poisoning often occurred for eating puffer fish. Currently main detection methods of tetrodotoxin are mouse assay and enzyme linked immunosorbent assay ( ELISA) etc. The tetrodotoxin detection methods presently need much more effort in time and cost, and can not meet the market requirements nowadays. We developed a tetrodotoxin colloidal gold immunochromato-graphic test kit, which the lowest detection limit was 100 ng/mL. The logic four parameter curve fitting, var-iation coefficient analysis, and standard test methods were used to test the stability, sensitivity, the fitting degree of the trend line, and detection range of homemade detection card in tetrodotoxin quantitative detec-ting. The results showed that the variation coefficient of homemade tetrodotoxin fluorescence immuno-chrom-atographic testing card was 0. 124; correlation coefficient r2 value was 0. 995 5; the detection range was 2~600 ng/mL. The testing card can meet the need of tetrodotoxin detection.关键词
河豚毒素/免疫荧光层析/快速定量检测Key words
tetrodotoxin/immunofluorescence chromatography/rapid quantitative detection分类
医药卫生引用本文复制引用
苏捷,钟建兴,李雷斌,吴靖娜,刘智禹..免疫荧光层析快速检测河豚毒素的研究[J].渔业研究,2016,38(6):460-465,6.基金项目
福建省海洋高新产业发展专项项目(闽海洋高新〔2013〕027号、闽海洋高新〔2016〕07号) （闽海洋高新〔2013〕027号、闽海洋高新〔2016〕07号）
厦门南方海洋研究中心项目(14GZP024NF24、14PZY017NF17) （14GZP024NF24、14PZY017NF17）
福建省海洋经济创新发展区域示范项目(2014FJPT01) （2014FJPT01）
