分析测试学报2016，Vol.35Issue(11)：1451-1455,5.DOI:10.3969/j.issn.1004-4957.2016.11.014
超声辅助分散液液微萃取-高效液相色谱测定护肤水中9种荧光增白剂
Simultaneous Determination of Nine Fluorescent Whitening Agents in Skincare Water Using Ultrasound-assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography
摘要
Abstract
A method based on ultrasonic-assisted dispersive emulsion microextraction procedure coupled to high performance liquid chromatography with fluorescence detector(UA-DLLME-HPLC-FLD) was developed for the determination of nine fluorescent whitening agents (FWAs) residues in skincare water.Experimental conditions affecting the microextraction procedure such as type and volume of extraction solvent,content of disperse solvent,salt concentration,pH value,extraction time and centrifugal time were investigated.The ultrasonic-assisted disperse emulsion microextraction conditions were optimized by using 50 μL chloroform as solvent and 250 μL methanol as disperser solvent,and the mixture was centrifuged at 4 500 r/min for 2 min.Under the optimum conditions,the calibration curves of nine fluorescent whitening agents in a certain concentration range with correlation coefficients more than 0.999 0.The limits of detection(LODs) of night fluorescent whitening agents ranged from 0.06 mg/kg to 4.20 mg/kg and the limits of quantitation(LOQs) ranged from 0.2 mg/kg to 14.0 mg/kg.The average recoveries for nine fluorescent whitening agents residues in skincare water were in the range of 84.3%-102.4%.Compared to the previous methods,the new procedure has the characteristics of simple sample preparation,low organic solvent consumption and high sensitivity.关键词
分散液液微萃取/超声辅助/高效液相色谱/荧光增白剂(FWAs)/护肤水Key words
dispersive liquid-liquid microextration(DLLME)/ultrasound-assisted(UA)/high performance liquid chromatography(HPLC)/fluorescent whitening agents(FWAs)/skincare water分类
化学化工引用本文复制引用
张宪臣,华洪波,薄艳娜,李蓉,张朋杰,杨芳,王勇,熊仁广..超声辅助分散液液微萃取-高效液相色谱测定护肤水中9种荧光增白剂[J].分析测试学报,2016,35(11):1451-1455,5.基金项目
国家质检总局科技计划项目(2015IK052) （2015IK052）
中山市科技计划项目(2014A2FC241) （2014A2FC241）
广东检验检疫局科技计划项目(2015GDK32) （2015GDK32）
