广西植物2016，Vol.36Issue(12)：1403-1409,7.DOI:10.11931/guihaia.gxzw201511023
五种金花茶组植物的耐寒性比较研究
Cold tolerance of five species of Camellia sect. Chrysantha
摘要
Abstract
Camellia sect. Chrysantha are world-famous economic and ornamental plants with high ornamental and medicinal values. In order to determine the cold tolerance, to lay a theoretical foundation for cold tolerance breeding and to provide a technical support for artificial cultivation of Camellia sect. Chrysantha, two-year old leaves of five species were employed as materials and treated under artificially simulated low temperature environment, including C. nitidissima, C. longzhouensis, C. limonia, C. multipetala and C. tunghinensis. We obtained the changes of electrolyte leakage rates (REC) at 20℃ (nor-mal temperature control), 8℃,-2℃,-7℃,-12℃,-17℃,-22℃ and-27℃ by using conductance method,calcu-lated the semi-lethal low temperatures ( LT50 ) based on the changes in electrolyte leakage rates ( REC) under different low temperature stresses with the Logistic equation, and also analyzed the contents of free proline, soluble sugar and malondial-dehyde ( MDA) in leaves. The results showed that the semi-lethal temperature ( LT50 ) of five species was:C. nitidissima-14.58℃, C. longzhouensis -14. 27 ℃, C. limonia -13. 44 ℃, C. multipetala -13. 09 ℃, and C. tunghinensis -12. 74 ℃. The cold tolerance of five species Camellia sect. Chrysantha could be reliably reflected by the semi-lethal temperature. C. nitidissima and C. longzhouensis had high cold tolerance. C. limonia and C. multipetala had medium cold tolerance. C. tunghinensis had poor cold tolerance. The REC of the five species increased following an S-curve with the tem-perature drop, and temperature showed highly significant negative correlation to cold tolerance. The contents of free proline, soluble protein and MDA increased first and then decreased. Under the same temperature treatment, C. nitidissima with low-er LT50 temperature had higher contents of free proline, soluble protein and lower contents of MDA. The results provide the information for selection of cold-tolerant germplasm of Camellia sect. Chrysantha.关键词
金花茶组/相对电导率/半致死温度/耐寒性Key words
Camellia sect. Chrysantha/relative electric conductivity/semi-lethal temperature/cold tolerance分类
农业科技引用本文复制引用
李吉涛,谢伟玲,柴胜丰,唐健民,韦霄..五种金花茶组植物的耐寒性比较研究[J].广西植物,2016,36(12):1403-1409,7.基金项目
国家自然科学基金(31160137) （31160137）
广西自然科学基金(2015GXNSFBA139051, 2016GXNSFAA380122) （2015GXNSFBA139051, 2016GXNSFAA380122）
广西植物研究所基本业务费项目(桂植业 14004 ) [ Supported by the National Natural Science Foundation of China ( 31160137 ) （桂植业 14004 ）
the Natural Science Foundation of Guangxi (2015GXNSFBA139051, 2016GXNSFAA380122) （2015GXNSFBA139051, 2016GXNSFAA380122）
the Science Research Foundation of Guangxi Institute of Botany (Guizhiye 14004)]. （Guizhiye 14004）
