广西植物2016，Vol.36Issue(12)：1432-1438,7.DOI:10.11931/guihaia.gxzw201512029
黑蒴定芽诱发增殖的初步研究
Adventitious bud induction and multiplication of Melasma arvense
摘要
Abstract
Melasma arvense is known as one of the precious herbs in Yunnan Province, and has the effects on leukemia, cancer, dispersing blood stasis and hemolysis. It has become a hot spot for the development of natural drugs in Yunnan. At present, because of its peculiar habitat, seed dispersal difficulty, reproductive barriers, seed germination and establishment difficulty and over-harvesting, wild plants are becoming extinctive recent years in Yunnan Province. Therefore, in order to realize resource protection and restoration, it is necessary to carry out resource conserva-tion and artificial breeding. In order to primary establish the adventitious bud induction and multiplication system that present excellent properties of growth and multiplication, used stem shoots as tissue culture inoculation materials studied the effects of different medium type, different concentrations of cytokinin and different natural organic additives on bud multiplication and growth based on the prophase research of provenance collection, artificial propagation and screening of different organ culture of M. arvense. The results indicated that about 2-3 cm long stem with buds cultured on culturalconditions(1/2 MS with 6-BA 0.5 mg·L-1+ Pt 50 g·L-1+ Bn 80 g·L-1+ 25 g·L-1 sugar + 7 g·L-1 agar with pH=5.8, a temperature of 22℃, light 10 h·d-1) for 8-10 d, they produced numerous adventitious buds. Each explant pro-duced 8.1 shoots and the grow length of main stem was 74.2 mm in 40 d. The experiment primarily established the adven-titious bud induction and multiplication system that present excellent properties of growth and multiplication. This study provides a scientific basis for the artificial cultivation and sustainable use.关键词
黑蒴/定芽诱发/组织培养/基本培养基/细胞分裂素/天然有机添加物Key words
Melasma arvense/induction of adventitious bud/tissue culture/basic medium/cytokinin/natural organic additives分类
农业科技引用本文复制引用
李育川,房海灵,王定康,向晓东,靳松,耿开友..黑蒴定芽诱发增殖的初步研究[J].广西植物,2016,36(12):1432-1438,7.基金项目
云南省高校优势特色重点学科建设项目(生态学) （生态学）
昆明学院人才引进项目(YJL11027)[Supported by Key Disciplines Project of Yunnan Education Department (Ecology) （YJL11027）
Kunming University of Talent Introduction Project(YJL11027)]. （YJL11027）
