广西植物2016，Vol.36Issue(12)：1460-1467,8.DOI:10.11931/guihaia.gxzw201510027
木薯华南8号组培苗对盐胁迫的生理响应
Physiological mechanism of cassava South China 8tissue culture seedling under salt stress
摘要
Abstract
The cassava ( Manihot esculenta) South China 8 ( SC8) tissue culture seedlings were conducted to investigate the physiological mechanism in response to salt stress. Effects of MS medium treatment contained NaCl ( 0, 5, 20, 35, 50 mmol·L-1 and R50 mmol·L-1 ) on the growth and physiological indexes activities of Cassava SC8 tissue culture seed-lings were evaluated. The physiological indexes include chlorophyll, hydrogen peroxide ( H2 O2 ) , malonaldehyde (MDA) content, and the activities of superoxide dismutases (SOD), catalases (CAT), peroxidase (POD), ascorbate peroxidase (APX). The results of physiological showed that the growth of SC8 tissue culture seedlings did not change significantly after NaCl content (≤20 mmol·L-1 ) stress for 60 d, but high content (≥35 mmol·L-1 ) of the stress had inhibited SC8 tissue culture seedlings growth. The growth of SC8 tissue culture seedlings could be recovered at NaCl con-cent (50 mmol·L-1) stress for 30 d after they were transferred to the normal MS medium of 30 d. Chlorophyll and MDA contents were accumulated at NaCl content(≤35 mmol·L-1 ) stress, but which were not accumulated at NaCl content (≥50 mmol·L-1 ) stress. H2 O2 content had no obvious change at NaCl content stress which it was compared with con-trol. Activities of antioxidant enzymes including POD, CAT and APX were increased at NaCl stress, whereas the activi-ties of SOD, CAT and APX were decreased at higher NaCl stress. The results of real-time quantitative PCR showed that the expression level of SOD, CAT, POD and APX were higher than control at NaCl content(≥50 mmol·L-1)stress. The results indicated that salt stress for a short period of time would not cause devastating damage to cassava, which could improve the salt tolerance of cassava by regulating the activity of physiological indexes.关键词
木薯/NaCl处理/生理指标测定/相关性分析/Q-PCRKey words
cassava/NaCl stress/physiological indexes measuring/correlation analysis/Q-PCR分类
农业科技引用本文复制引用
薛晶晶,朱文丽,陈松笔..木薯华南8号组培苗对盐胁迫的生理响应[J].广西植物,2016,36(12):1460-1467,8.基金项目
国家"十二五"农村领域科技计划项目 (2012AA101204-2) （2012AA101204-2）
海南省高层次创新创业人才启动基金 （）
中央级公益性科研院所基本科研业务费专项(1630032013005) [Supported by National Scientific and Technological Programs in Rural Fields(2012AA101204-2) （1630032013005）
the Initial Fund of High-levele Creative Talents in Hainan Province （）
the National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032013005)]. （1630032013005）
