广西植物2016，Vol.36Issue(12)：1483-1491,1482,10.DOI:10.11931/guihaia.gxzw201603001
镉胁迫下紫花苜蓿幼苗内源一氧化氮和活性氧的生成
Effects of endogenous NO and ROS generation in alfalfa under Cd stress
摘要
Abstract
Seedlings of alfalfa variety Medicago sativa cv. Gannong No. 3 were grown in nutrient solution with the Cd concentration between 0 to 2.0 mmol·L-1 . The generation patterns of endogenous nitric oxide ( NO) and reactive oxygen species ( ROS) in roots, stems and leaves were studied, and the changes of root activity was determined. The results showed that NO content in seedlings presented a tendency of increasing first and decreasing afterwards, finally recovering normal with increasing concentration of Cd from 0 to 2.0 mmol·L-1 . The NO content was influenced significantly by ni-tric oxide synthase ( NOS) and nitrate reductase ( NR) in stems, while NOS, nitrite ions ( NO2-) and NR determined the NO content in leaves. Similarly, NOS and NO2- had great relevance with NO content in roots. The increase of Cdconcentration in solution leaded to the significantly rising of content of hydrogen peroxide ( H2 O2 ) , malondialdehyde (MDA), superoxide anion (O-2·) and relative electric conductivity (REC) in seedlings, indicating that the stress of high Cd concentration made the ROS vast accumulated, the cell membrane damaged, and cytoplasm outflowed, further caused membrane lipid peroxidation. Besides, there was no significant correlation between NO and ROS due to their dif-ferent mechanisms of generation.关键词
镉胁迫/紫花苜蓿/一氧化氮/活性氧Key words
cadmium stress/alfalfa ( Medicogo sativa)/nitric oxide/reactive oxygen species分类
农业科技引用本文复制引用
蘧苗苗,陈银萍,苏向楠,NGABIRE Maurice,杨波,柯昀琪,MUKUNDWA Anne Marie..镉胁迫下紫花苜蓿幼苗内源一氧化氮和活性氧的生成[J].广西植物,2016,36(12):1483-1491,1482,10.基金项目
国家自然科学基金(31260089,31560161,31170413)[Supported by the National Natural Science Foundation of China(31260089,31560161, 31170413) ]. （31260089,31560161,31170413）
