广西植物2016，Vol.36Issue(12)：1498-1504,1467,8.DOI:10.11931/guihaia.gxzw201510024
转基因烟草在PEG6000模拟干旱胁迫条件下的生理响应
Physiological response of transgenic tobacco to drought stress simulated by PEG6000
摘要
Abstract
Chorispora bungeana is a rare alpine subnival plant species, the extremely harsh living environment makes it become a new plant for study the responses of drought stress. In this study we used CbPLDα, CbPLDβtransgenic tobacco plants as material to find the mechanism of plants response drought stress, and we also detected the osmolytes content and protective enzymes activity in CbPLDα, CbPLDβtransgenic tobacco plants, using the ways of the drought stress sim-ulated by PEG6000. The results showed that proline, solube sugar and soluble protein involved in strengthening resist-ance of CbPLDα, CbPLDβtransgenic tobacco to drought stress simulated by PEG6000,and in different concentrations of PEG6000 simulate drought stress, CbPLDα, CbPLDβtransgenic tobacco proline, soluble sugar and soluble protein con-tent were always significantly higher than that of wild type tobacco plants ( P<0.05) . This indicated that osmotic adjust-ment ability of drought stress in CbPLDαand CbPLDβtransgenic tobacco was stronger than the wild type tobacco plants.In the protective enzymes, Superoxide dismutase(SOD) and Peroxidase (POD) have significant complementary effects on mitigating the injury of membrane lipid peroxidation of transgenic tobacco;Catalase ( CAT) and Ascorbate peroxidase ( APX) played major roles in the mechanism of removal of hydrogen peroxide. SOD,POD,CAT and APX had higher ac-tivity in CbPLDα, CbPLDβtransgenic tobaccos than in wild tobacco plants ( P<0.05) . All of these results indicated that protective enzymes had important biological function in protecting transgenic tobacco from drought stress simulate by PEG6000. It partly revealed the physiological and ecological mechanisms of the CbPLDα, CbPLDβgenes responding to drought environment in C. bungeana. The results showed that both CbPLDαand CbPLDβtransgenic tobaccos involved in the regulation pathway of membrane stability, osmotic adjustment and the regulation of anti-oxidative system. CbPLDαand CbPLDβ genes can provide powerful genetic resources for improving drought resistance of plants. The results strengthen the PLD for functional studies, supplementary plant resistance to drought stress and drought resistance theory breeding germplasm development and utilization, has important theoretical value and practical significance.关键词
PEG6000模拟干旱胁迫/CbPLDα/CbPLDβ/渗透调节物质/保护酶Key words
drought stress simulated by PEG6000/CbPLDαand CbPLDβ/osmolytes/protective enzyme分类
农业科技引用本文复制引用
陈霞,杨鹏军,张旭强,杨宁..转基因烟草在PEG6000模拟干旱胁迫条件下的生理响应[J].广西植物,2016,36(12):1498-1504,1467,8.基金项目
国家自然科学基金(31160087, 31360061)[Supported by the National Natural Science Foundation of China (31160087, 31360061)]. （31160087, 31360061）
