化工进展2016，Vol.35Issue(z2)：319-323,5.DOI:10.16085/j.issn.1000-6613.2016.s2.056
杜仲中环烯醚萜类物质对性激素转化的调控作用
Regulation effects of eucommia iridoids on sex hormone transformation
摘要
Abstract
Eucommia bark is a rare traditional Chinese tonic herb,benefiting liver and kidney, strengthening tendons and bones,and preventing miscarriage.However,its effects are closely related to sex hormones, so the effects of three main iridoids of Eucommia (genipiin, geniposide,and geniposidic acid ) on sex hormones ’ transformation were studied in this paper.KGN,a tumor ovarian granulosa cell line was chosen as the transformation system of hormone, pregnenolone as conversion substrate, progesterone, testosterone, and estradiol levels in the culture medium were measured respectively after interfered by the iridoids for 24 hours.Moreover,cell RNA was also collected to study the catalytic enzymes’expression levels (3β-HSD, CYP17A1, and 17β-HSD ) by reverse transcription and real-time fluorescent quantitation method.The research results showed that 50 μmol/L genipin could significantly improve 3β-HSD,CYP17A1,and 17β-HSD expression levels,and significantly promote the synthesis of testosterone and estradiol further.关键词
杜仲/环烯醚萜/性激素/芳香化酶/转化Key words
Eucommia/iri doid/sex hormone/aromatase/transformation分类
化学化工引用本文复制引用
左涛,宋航..杜仲中环烯醚萜类物质对性激素转化的调控作用[J].化工进展,2016,35(z2):319-323,5.基金项目
成都市科技局项目(2014-HM01-00247-SF)。 （2014-HM01-00247-SF）
