海洋科学2016，Vol.40Issue(10)：16-24,9.DOI:10.11759//hykx20150917002
暗纹东方鲀生长性状相关微卫星标记筛选
Screening growth-related microsatellite markers inTakifugu obscurus
摘要
Abstract
A population of Takifugu obscurus with significant growth differences was investigated for screening molecular markers linked to growth traits, using SSR combined with bulked segregation analysis (BSA). Two gene pools were respectively constructed using 30 DNA templates isolated fromT. obscurus samples with significantly different growth rates and then amplified by 85 pairs of microsatellite primers. The unique alleles were found in 14 loci. These 60T. obscurus individuals were genotyped by SSRs. The results showed that loci TOP03, TOG01, fms15, and fms75 had a highly significant negative correlation with growth trait (P < 0.01), while fms89 showed extremely significant positive correlation with growth trait (P < 0.01). The verification test using another population of 30 randomly selectedT. obscurus with significantly different growth rates indicated that only loci fms15 and fms75 were significantly correlated with growth trait, with correlation coefficients of –0.384 and –0.411, respectively. Therefore, these two microsatellite loci with a significant linkage with growth traits could be used as reference markers for marker-assisted breeding ofT. obscurus.关键词
暗纹东方鲀(Takifugu obscurus)/生长性状/微卫星/相关性分析Key words
Takifugu obscurus/growth trait/microsatellite/correlation analysis分类
农业科技引用本文复制引用
马爱军,邹杰,孙建华,王婷,王广宁,崔文晓,王新安,刘大勇,郭正龙..暗纹东方鲀生长性状相关微卫星标记筛选[J].海洋科学,2016,40(10):16-24,9.基金项目
江苏省科技支撑计划资助项目(BE2013345) （BE2013345）
江苏省水产三新工程资助项目(Y2013-12)Jiangsu Science and Technology Support Program, No. BE2013345 （Y2013-12）
Jiangsu Aquatic Three New Projects , No. Y2013-12 （）
