海洋科学2016，Vol.40Issue(10)：113-119,7.DOI:10.11759/hykx20151225002
高效液相色谱-离子阱质谱法测定海洋微藻藻粉中的8种脂溶性毒素
Determination of eight typical lipophilic algae toxins in marine microalgae powder using high-performance liquid chromatography- ion trap mass spectrometry
摘要
Abstract
A method involving high-performance liquid chromatography coupled with electrospray ionization–ion trap mass spectrometry (HPLC–ESI–IT–MS) was developed to determine eight typical lipophilic algae toxins (okadaic acid (OA), dinophysistoxin-1 (DTX-1), pectenotoxin-2 (PTX-2), yessotoxin (YTX), azaspiracid1, 2 (AZA1,AZA2), spirolidel1 (SPX1), gymnodimine (GYM)) in marine microalgae powder. The powder was extracted using ultrasound-assisted extraction after the algae cell-breaking process was performed using ultrasonication. The crude extracts were then analyzed using HPLC–ESI–IT–MS in the multiple reaction monitoring mode. The results showed that the calibration curve for each lipophilic algae toxin was obtained with a good correlation coefficient (R2>0.991)in its linear concentration. The detection limits of the eight target toxins ranged from 0.85 to 1.315 pg. The average recoveries of these toxins ranged from 88.5% to 111.4% with relative standard deviations in the range of 4.82%–10.17%. The proposed method was applied to determine typical lipophilic algae toxins in marine microalgae powder, and good results were obtained. The results also indicated that this method is a useful tool for determining lipophilic algae toxins in marine microalgae powder.关键词
高效液相色谱-离子阱质谱/腹泻性贝毒/海洋微藻Key words
High-performance liquid chromatography-ion trap mass spectrometry/Diarrhetic shellfish poisoning/Marine microalgae分类
生物科学引用本文复制引用
高莉媛,王艳龙,陈军辉,王帅,史小星,石洪华,郑立..高效液相色谱-离子阱质谱法测定海洋微藻藻粉中的8种脂溶性毒素[J].海洋科学,2016,40(10):113-119,7.基金项目
山东省自然科学基金(ZR2015PD003) （ZR2015PD003）
国家基金委-山东省联合基金项目(U1406403) （U1406403）
国家科技支撑计划项目(2013BAK12B00)Shandong Provincial Natural Science Foundation, No.ZR2015PD003 （2013BAK12B00）
National Natural Science Foundation of China-Shandong Joint Funded Project, No.U1406403 （）
National Science & Technology Pillar Program of China, No.2013BAK12B00 （）
