健康研究2016，Vol.36Issue(6)：627-629,633,4.DOI:10.3969/j.issn.1674-6449.2016.06.009
miR-100抑制胃癌细胞裸鼠移植瘤的生长及机制的研究
Effect of MiR-100 in inhibiting the growth of human gastric cancer in nude mice
摘要
Abstract
Objective To examine the mechanism of miR-100 in inhibiting the growth of human gastric cancinoma SGC-7901 cell in nude mice .Method The nude mice as subjects of the study were first randomly divided into 2 groups:mir-100 group ( experimental group ) and negative control group .In order to establish the model , SGC7901 cells transfected with GFP-miR-100 or GFP-miR-negative were injected into the axillary of nude mice of the two groups respectively .The expression of in vivo mir-100 by GFP image, lung metastases and stromal infiltration were observed by HE staining . Immunohistochemistry and Western blot were used to detect the expression of target gene of miR -100 .Findings miR-100 was overexpressed stably in the experimental group .Six of the eight injected mice in the control group had cancer cells invading the stroma surrounding the tumors .In stark contrast , stromal invasion was only found in two of the nine injected mice in the experimental group .The tumor nodules of the remaining 7 mice were well-encapsulated by intact fibrotic capsules.Western blot and immunohistochemistry showed that miR-100 effectively inhibited the expression of ZBTB 7A protein in xenograft tumor of nude mice .Conclusion miR-100 inhibits the metastasis and growth of human gastric cancer in nude mice by inhibiting the ZBTB7A target gene.关键词
miR-100/胃癌/裸鼠细胞瘤Key words
MiR-100/gastric cancer/cell xenograft in nude mice分类
医药卫生引用本文复制引用
方莹,张小平,邬万新..miR-100抑制胃癌细胞裸鼠移植瘤的生长及机制的研究[J].健康研究,2016,36(6):627-629,633,4.基金项目
嘉兴市科技局项目（2015c23011）；嘉兴市病理科重点学科 （）
