江苏大学学报（医学版）2016，Vol.26Issue(6)：475-479,483,6.DOI:10.13312/j.issn.1671-7783.y160223
长链非编码 RNA H19对胰腺癌细胞增殖、侵袭及转移的影响
Effects of long non-coding RNA H19 expression on cell proliferation, migration and invasion of pancreatic cancer cells
摘要
Abstract
Objective:To investigate the effects of long-coding RNA H19 on proliferation,migration and invasion of pancreatic cancer cells.Methods:We first detected the expression of H19 in Patu8988, SW1990,Bxpc3 and Panc-1 pancreatic cancer cells with real-time quantitative PCR (qRT-PCR).The SW1990 and Bxpc3 cells had higher H19 expression while Patu8988 and Panc-1 had lower H19 expression. The H19 siRNAs were transfected into pancreatic cancer cells SW1990 in vitro.The mRNA expression of ZEB1 and mir-200 were analyzed with qRT-PCR.The effects of H19 on proliferation were evaluated by CCK-8 and colon formation assays.The expression of cell proliferation-and EMT-related proteins were de-tected with Western blotting.Results:Compared with the control group,SW1990 cells transfected with H19 siRNAs revealed a significant lower level of H19 and lower the ability of cell colony formation.Howev-er,there was no significant change on cell proliferation rate;the expressional levels of ZEB1,Snail and PC-NA were up-regulated as well as the ratio of Bcl-2 to Bax;the mRNA level of ZEB1 was up-regulated while the expression of miR-200 showed no significant change.Conclusion:Down-regulation of H19 promotes cell proliferation,migration and invasion in pancreatic cancer cells SW1990.关键词
H19/ZEB1/mir-200/细胞侵袭/细胞转移Key words
H19/ZEB1/mir-200/cell migration/cell invasion分类
医药卫生引用本文复制引用
王珏,张尤历,徐岷,张行行,王文兵..长链非编码 RNA H19对胰腺癌细胞增殖、侵袭及转移的影响[J].江苏大学学报（医学版）,2016,26(6):475-479,483,6.基金项目
国家自然科学基金资助项目（81472333，81672402）；江苏省自然科学基金资助项目（BK20131247）；江苏省研究生创新计划 （）
