河南科技大学学报（医学版）2016，Vol.34Issue(4)：259-261,3.DOI:10.15926/j.cnki.issn1672-688x.2016.04.006
HPVL1及P16蛋白在诊断宫颈鳞状上皮病变的应用及价值
Value of Diagnosis Cervical Squamous Epithelial Lesions with HPV L1 and P16 Protein
黄建伟 1田学智 1张王娜 1冯阳阳 1叶婷 1魏素娇1
作者信息
- 1. 郑州大学附属洛阳市中心医院病理科，河南洛阳471000
- 折叠
摘要
Abstract
Objective To investigate the expression of major capsid protein L1 and P16 protein in the human papilloma virus ( HPV) , and determine its value in the diagnosis and application of cervical squamous epithelial lesions. Methods 215 cases of high risk with HPV positive expression were collected from Luoyang Central Hospital, whicht including 101 cases of chronic cervicitis, 60 cases of CIN grade Ⅰ, 27 cases of CIN grade Ⅱ, 21 cases of CIN grade Ⅲ and 6 cases of squamous cell carcinoma ( SCC ) . Immunohistochemical staining was used to detect the expression of HPV L1 and P16 protein of all cases. Results The expression rate of HPVL1 protein in cervicitis, CIN Ⅰ, CIN Ⅱ, CIN Ⅲ and SCC were 66. 33%(67/101), 71. 67%(43/60), 25. 92%(7/27), 9. 52%(2/21) and 0%(0/6), respectively. The expression rate of P16 protein in cervicitis, CINⅠ, CINⅡ, CINⅢand SCC were 69. 31%(70/101), 58. 33%(35/60), 85. 19%(23/27), 95. 24%(20/21) and 100%(6/6) , respectively. The rate of expression of HPVL1 protein in cervical intraepithelial lesions ( CIN Ⅰ and CIN Ⅱ, CIN Ⅲ, SCC ) decreased with the increasing of pathological grade of cervical epithelium ( P <0 . 05 ) , but there was no significant difference between cervicitis and CIN I (P>0. 05). On the contrary, the expression rate of P16 in cervical intraepithelial lesions increased with the increasing of pathological grade. It was a negative correlation between the expression of the two proteins (P<0. 05). Conclusion Combined detection of HPV L1 and P16 protein had a certain diagnostic value in cervical disease screening with HPV positive patients.关键词
人乳头状瘤病毒/免疫组化/宫颈上皮病变Key words
human papilloma virus/immunohistochemistry/cervical intraepithelial neoplasia分类
医药卫生引用本文复制引用
黄建伟,田学智,张王娜,冯阳阳,叶婷,魏素娇..HPVL1及P16蛋白在诊断宫颈鳞状上皮病变的应用及价值[J].河南科技大学学报（医学版）,2016,34(4):259-261,3.
