河南科技大学学报（医学版）2016，Vol.34Issue(4)：286-288,3.DOI:10.15926/j.cnki.issn1672-688x.2016.04.015
洛阳地区常见嗜尸性丽蝇的COI序列分析
CO I Sequence Analysis of Common Calliphoridae Necrophagous Flies from Luoyang
摘要
Abstract
Objective To analyze the common Calliphoridae flies from Luoyang by high polymorphisms of mtDNA CO I gene for identifying the species of necrophagous flies. Methods Eight flies including C. Megacephala and L. sericata were collected and classified by entomologists with traditional morphological characteristics. The DNA of flies was extracted with Chelex-100 method. The fragments of CO I were amplified and sequenced. All the sequences were analyzed by related software packages for nucleotide composition, genetic distance computation and phylogenetic tree construction. Results The result of amplification with 8 samples showed that length of the obtained CO I sequences were 647 bp. The interspecific difference in CO I ranged from 0 . 114 to 0 . 117 with an average of 0 . 116 , while the intraspecific difference was 0 to 0 . 002 . Conclusion The results indicated that congeneric species could be separated by CO I gene, which could be a supplement of necrophagous flie gene pool.关键词
辅酶I/嗜尸性蝇类/双翅目丽蝇科/序列分析/法医昆虫学Key words
coenzymeⅠ/necrophagous flies/Diptera Calliphoridae/sequence analysis/forensic entomology分类
生物科学引用本文复制引用
赵琳琳,翟仙敦,张振,吕宙,夏志远,莫耀南..洛阳地区常见嗜尸性丽蝇的COI序列分析[J].河南科技大学学报（医学版）,2016,34(4):286-288,3.基金项目
河南省基础与前沿课题(112300410082)河南科技大学基金(09001309,13000914,13000696) （112300410082）
