河南科技大学学报（医学版）2016，Vol.34Issue(4)：310-315,6.DOI:10.15926/j.cnki.issn1672-688x.2016.04.022
人外周血基因组DNA提取的影响因素及方案选择
Influential Factor and Protocols Selection for Genomic DNA Extraction from Human Peripheral Blood Samples
摘要
Abstract
Objective Human peripheral blood samples were one of the main sources used to obtain genomic deoxyribonucleic acid ( gDNA) . In order to select the optimal DNA extraction protocol on forensic identification, it would be helpful to investigate the research progress of DNA extraction. Methods There reviewed the common procedures adopted by most DNA extraction protocols, and highlighted the principles of its operation and impact factors by searching the representative studies from database. Results The lysis of white blood cell ( WBC) was a key step to determine the yield and quality of extracted DNA. Detergents were usually the core component of a variety of WBC lysis solution. Another key step was the separation and purification of free DNA which usually involved some solid media. The manual protocols had still common alternatives for DNA extraction from peripheral blood samples, especially when a few samples were processed in small laboratories. A variety of DNA extraction kits had been widely used at present. Several automated systems for gDNA extraction were very suitable for treating the large quantities of blood samples. However, there was not enough scientific evidence to support one particular DNA extraction method from whole blood samples in terms of DNA yield and quality, time consumption, safety, and cost. Conclusion The optimal choice for gDNA extraction had still based on comprehensive consideration of multiple items, such as DNA extraction efficiency, time consumption, safety, cost, and availability of equipment and reagents from variety protocols.关键词
外周血/基因组DNA/DNA提取Key words
peripheral blood/genomic DNA/DNA extraction分类
社会科学引用本文复制引用
张振,杨文涛,陈炯,冯巍..人外周血基因组DNA提取的影响因素及方案选择[J].河南科技大学学报（医学版）,2016,34(4):310-315,6.基金项目
国家自然科学基金(81501634)河南科技大学高级别项目培育基金(012ZCX021) （81501634）
