特产研究2016，Vol.38Issue(4)：40-43,4.DOI:10.16720/j.cnki.tcyj.2016.04.010
高效凝胶渗透色谱法测定凝血酶冻干粉的分子量及其分布
Determination of Molecular Weight and its Distribution of Lyophilizing Thrombin Powder by HPGPC
摘要
Abstract
To establish a quantitative method for molecular weight in Lyophilizing Thrombin Powder .The determination was carried out using a TSK GEL G2000 SW (7.5mm × 300mm ,10μm ) .The mobile phase consisted of pH 6.86 phosphate buffer at a flow rate of 0.5mL/min .The de-tection device was a UV at the detection detector ,wavelengths of 280nm .Molecular weight of Lyophilizing Thrombin Powder is 80ku-95ku . The quantitative method was set up by protein molecular weight .The method is rapid ,simple and good reproducibility ,and can provide the basis for the improvement and optimization of the quality standard of Lyophilizing Thrombin Powder .It can be used as a fingerprint chromatogram from the perspective of protein peptide molecular weight to control the immanent quality of Lyophilizing Thrombin Powder .关键词
高效凝胶渗透色谱法/重均分子量/凝血酶冻干粉Key words
HPGPC/weight-average molecular weight/lyophilizing thrombin powder分类
医药卫生引用本文复制引用
陈英红,王颖,姜瑞芝,张晓荧..高效凝胶渗透色谱法测定凝血酶冻干粉的分子量及其分布[J].特产研究,2016,38(4):40-43,4.基金项目
吉林省科技发展计划项目 （）
