应用化学2016，Vol.33Issue(12)：1355-1365,11.DOI:10.11944/j.issn.1000-0518.2016.12.160147
小分子与三链 DNA相互作用的研究进展
Research Progress on Interaction Between Small Ligands and Triplex DNA
摘要
Abstract
Triplex DNA can interfere with gene transcription and expression modulation .It is one kind of important biological macromolecules .The interaction between small molecules and DNA targets plays an important role in the function of drug mediated physiological processes .In the past decades, numerous attempts have been made to screen triplex DNA binders .However, not too many effective methods for screening triplex DNA binders were reported . Basic principles , the screening methods of research and application for the interaction between small ligands and triplex DNA were summarized , including mainly the principle and application of several methods , such as competition dialysis , gold nanoparticle based approach , thermal denaturation method , centrifugal ultrafiltration method , electrospray ionization mass spectroscopy ( MS) method, and liquid chromatography-mass spectroscopy ( LC-MS) combined method .This review has a general instruction to the research of the interaction between small ligands and triplex DNA .关键词
小分子配体/三链DNA/稳定性/筛选方法/应用Key words
ligands/triplex DNA/stability/screening method/application分类
化学化工引用本文复制引用
杨洪梅,徐牛生,姚文斌,石磊,李金英,刘淑莹..小分子与三链 DNA相互作用的研究进展[J].应用化学,2016,33(12):1355-1365,11.基金项目
国家自然科学基金项目（21305135）和吉林省科技发展计划项目（20160101220JC）资助@@@@Supported by the National Natural Science Foundation of China（No．21305135）， the Science and Technology Development Planning Project of Jilin Province （）
