中国实验动物学报2016，Vol.24Issue(6)：558-566,9.DOI:10.3969/j.issn.1005-4847.2016.06.002
蕨麻小型猪心梗模型的建立及梗死边缘区的基因表达谱分析
Establishment of a myocardial infarction model and the gene expression profile in peri-infarct area in Juema minipigs
摘要
Abstract
Objective To establish a Juema minipig model of myocardial infarction, to evaluate the clinical indi⁃ces in the model pigs, and to explore the relationship between gene expression and metabolic decompensation. Methods 13 male Juema minipigs were randomly divided into control (Sham, n=5), myocardial infarction (MI, n=5) and normal control (for evaluating the recovery condition after surgery, n=3) groups. In the MI group, the ligation was done at the left descending coronary artery around the 1/3 distance to heart apex. Four weeks after the surgery, the cardiac function and serum biochemistry was analyzed. The histological changes and gene expression profiles in the myocardium in the peri⁃infarct area were exanimated. Results Ultrasonic images showed that the infarction was formed, the ejection fraction and fraction shortening were significantly reduced in the MI group ( ~32% and ~40% less than those of the sham group). Histological examination showed that myocardial fibers at the peri⁃infarct area were broken, dissolved, and there was con⁃nective tissue hyperplasia with increased neutrophil and lymphocyte infiltration. Microarray analysis revealed that two myo⁃cardial remodeling and pathology mediating pathways, three inflammation⁃related pathways, and 8 metabolic pathways ( in⁃cluding fatty acid, amino acid, and glucose metabolic pathways) were significantly changed. Conclusions We have suc⁃cessfully established a Juema minipig model of myocardial infarction. The less branches of the left descending coronary ar⁃tery allow us to establish a stable model by surgery with comparable characteristics in the clinic indices. The results of this study provides useful reference characteristics of an animal model with characteristic changes in the peri⁃infarct area.关键词
蕨麻小型猪/心肌梗死模型/模型评价/梗死边缘区/基因表达谱Key words
Juema minipig/Myocardial infarction model/Model evaluation/Peri-infarct area/Gene expression profile分类
生物科学引用本文复制引用
谭伟江,王剑,陈小红,王希龙,杨丰华,黄韧,王静,李想,李小辉,雷迁,黄家园,龚宝勇,李菁,陈智英..蕨麻小型猪心梗模型的建立及梗死边缘区的基因表达谱分析[J].中国实验动物学报,2016,24(6):558-566,9.基金项目
广东省科技专项（No．2016A020216019）；广东省实验动物重点实验室（No．2013A061402001）；国家自然科学基金－面上项目（No．31572351）；广东省科技专项（No．2015A030302035）；国家自然科学基金－青年科学基金项目（No．81500281）。 （）
