中国实验动物学报2016，Vol.24Issue(6)：628-631,4.DOI:10.3969/j.issn.1005-4847.2016.06.014
西藏小型猪IGF-1基因在不同生长发育阶段和不同组织器官中的差异表达
Differential IGF-1 gene expression in various tissues at different developmental stages in Tibet minipigs
摘要
Abstract
Objective To determine the expression and distribution of IGF⁃1 gene in different tissues of Tibet minipigs at different ages. Methods The changes of IGF⁃1 gene expression in different tissues and different growth stages of Tibet minipigs were detected by quantitative real⁃time fluorescence PCR technology and GAPDH gene was used as the reference. Results (1) The study of sequential expression of IGF⁃1 gene showed that the lowest expression of IGF⁃1 gene in muscle tissue was at 0, 0�25, 0�5, 1, and 2 years of age, whereas the highest expression of IGF⁃1 gene in skin tissue was at 0, 0�25, 0�5 years of age and the highest expression of IGF⁃1 gene in liver tissue was at 0�1, 2, 3 years of age. (2) IGF⁃1 gene was expressed in all the 7 examined tissues, and its expression level was in the following decreasing order:skin, lung, liver, kidney, spleen, heart and muscle at 0�25 years of age. The expression of IGF⁃1 gene in the skin was significantly higher than in other organ tissues (P<0�01). Conclusions The expression of IGF⁃1 gene of Tibet minipigs showed obvious temporal and spatial specificity.关键词
IGF-1基因/西藏小型猪/实时荧光定量PCRKey words
IGF-1 gene/Tibet minipigs/Quantitative real-time PCR分类
生物科学引用本文复制引用
田雨光,王玉珏,庞炜,岳敏..西藏小型猪IGF-1基因在不同生长发育阶段和不同组织器官中的差异表达[J].中国实验动物学报,2016,24(6):628-631,4.基金项目
2015科研助手项目（C1032246）；广东省医学科研基金（编号A2016255）；广东省科技计划项目（编号2016A030303008）。 （）
