中国实验动物学报2016，Vol.24Issue(6)：654-658,5.DOI:10.3969/j.issn.1005-4847.2016.06.019
苯污染对大鼠肝肾功能及血核苷酸水平的影响
Effects of benzene pollution on the liver and kidney function and blood nucleotide level in rats
摘要
Abstract
Objective The aim of the present study is to investigate the effect of benzene on the liver and kidney function and blood nucleotide level in Wistar rats. Methods The Wistar rats were treated with benzene (0�19, 0�38, 0�76 g/kg body weight) for 21 days. Different blood components were detected by an automatic biochemical analyzer. The blood cyclic adenosine monophosphate ( cAMP ) , cyclic guanosine monophosphate ( cGMP ) and heat shock protein⁃70 (HSP⁃70) levels were determined by enzyme linked immunosorbent assay method (ELISA). Results When Wistar rats were administered with benzene at the of 0�19, 0�38, or 0�76 g/kg body weight for 21 days, the total serum bilirubin level was increased than the control group by 134�40%, 173�63% and 254�75%, respectively; aspertate aminotransferase ( AST) was increased by 70�76%, 85�44% and 106�61%, creatinine was increased by 24�54%, 67�46% and 84�50%, creatine kinase was increased by 151�35%, 180�85% and 245�54%, urea (0�38 and 0�76 g/kg body weight groups) was increased by 0�48% and 23�43%; alanine transaminase level exceeded the upper limit of the readings, (1185�60%);cAMP level was increased by 41�84%, 264�02% and 314�23%, respectively, cGMP was increased by 31�29%, 40�46% and 272�14%, HSP⁃70 was increased by 82�11%, 187�37% and 1484�21%;amylase level was re⁃duced by 62�03%, 63�66% and 62�03%, respectively, than the control group. Conclusion Our results demonstrate that benzene pollution affects the plasma cAMP and cGMP ratio, causing metabolic changes even structural damages in liver and kidney function.关键词
苯/血液/环磷酸腺苷/环磷酸鸟苷/热休克蛋白-70/Wistar大鼠Key words
Benzene/Blood/cAMP/cGMP/HSP-70/Wistar rats分类
生物科学引用本文复制引用
俞发荣,杨博,李登楼,张诗爽,薛源,连秀珍,谢明仁..苯污染对大鼠肝肾功能及血核苷酸水平的影响[J].中国实验动物学报,2016,24(6):654-658,5.基金项目
国家自然科学基金项目（编号31060283）；甘肃省基础研究创新群体项目（编号145RJIA333）；兰州市科技计划项目（2014－1－182；2015－3－80）；甘肃省教育厅创新团队项目；西北民族地区侦查理论与实务研究中心资助；兰州市人才创新创业项目；甘肃政法学院重大科研项目。 （）
