中国比较医学杂志2016，Vol.26Issue(12)：45-50,6.DOI:10.3969/j.issn.1671-7856.2016.12.009
实验性干眼大鼠的角结膜上皮组织学改变
Morphological changes of corneal and conjunctival epithelia in a rat model of dry eye
张慧芹 1李颖 2许永根1
作者信息
- 1. 北京大学第三医院眼科中心，北京 100091
- 2. 沧州市中心医院眼一科，沧州 061000
- 折叠
摘要
Abstract
Objective To evaluate the rat model of dry eye induced by topical administration of benzalkonium chloride and compare the morphological characteristics of corneal and conjunctival tissues of the normal and model rats . Methods Topical administration of benzalkonium chloride (0.3%) twice per day for seven days to induce the rat model of dry eye.Pathology with HE and periodic acid-Schiff staining and transmission electron microscopy were performed to evaluate the corneal and conjunctival changes .Results The corneal and conjunctival epithelial cells were sloughed off , the number of goblet cells was decreased in the dry eye model .Microvilli were obviously lost in the surface epithelial cells of the dry eye rat cornea and conjunctiva compared with the normal rats , as indicated by the amount and size of microvilli . Conclusions The changes in the corneal and conjunctival epithelia of BAC-induced rat dry eye are similar to the changes in human dry eye .This rat model may play an essential role for dry eye studies in the future .关键词
干眼/苯扎氯铵/上皮细胞/超微结构Key words
Dry eye/Benzalkonium chloride/Epithelial cells/Cornea/Conjunctive/Pathology/Ultrastructure/Rat分类
医药卫生引用本文复制引用
张慧芹,李颖,许永根..实验性干眼大鼠的角结膜上皮组织学改变[J].中国比较医学杂志,2016,26(12):45-50,6.
