中国畜牧兽医2016，Vol.43Issue(11)：2803-2810,8.DOI:10.16431/j.cnki.1671-7236.2016.11.001
蓖麻蛋白对人外周血B淋巴细胞的作用及相关基因表达的研究
Study on the Effect of Ricin on Human Peripheral Blood B Lymphocyte and the Expression of Related Genes
摘要
Abstract
This study was amied to learn the toxic effects of ricin on human peripheral blood B lymphocytes (IM-9) and the expression of related genes.The extracted ricin of different concentrations were added to culture cells for 6 and 12 h to make sure the median inhibitory concentration,then culture cells at the median inhibitory concentration for 2,6,10 and 12 h,at each time point detect the expression of immune related genes CD40,IL-1β,TNF-α and apoptosis related genes Bcl-2,Bax,Caspase-3.The results showed that the inhibitory effect of ricin on IM-9 cells was increased with the increase of concentration and time.The expression of TNF-α and CD40 genes increased significantly in experimental group than control group at 6 h (P<0.05).10 and 12 h reached extremely significant level (P<0.01);There was no significance of IL-1β gene expression between experimental and control groups (P>0.05).About apoptosis gene,the Bax gene expression decreased extremely significantly in experimental group at 10 h (P<0.05) and decreased significantly at 12 h (P<0.05);Bcl-2 gene had reached significant levels of four time periods (P<0.05), and 2,12 h were extremely significant (P<0.01);The expression of Caspase-3 reduced at 6 h,the other time points increased significantly (P<0.05),at 2 h reached extremely significant level (P<0.01).It showed that the gene expression of IM-9 cells could be significantly affected by ricin,TNF-α and CD40 genes were two potential genes to evaluate the toxicity by IM-9 cells.关键词
蓖麻蛋白/外周血B淋巴细胞/基因表达Key words
ricin/peripheral blood B lymphocyte/gene expression分类
生物科学引用本文复制引用
甄二东,黄素娟,化朝举,陶聪,杨述林,王彦芳,周荣,敖红,李奎..蓖麻蛋白对人外周血B淋巴细胞的作用及相关基因表达的研究[J].中国畜牧兽医,2016,43(11):2803-2810,8.基金项目
转基因新品种培育重大专项(2014ZX08011-006) （2014ZX08011-006）
中国农业科学院科技创新工程(ASTIP-IAS05) （ASTIP-IAS05）
