中国畜牧兽医2016，Vol.43Issue(11)：2835-2843,9.DOI:10.16431/j.cnki.1671-7236.2016.11.005
牛乳头状瘤病毒贵州株L1基因克隆及生物信息学分析
Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province
摘要
Abstract
In order to study and analyze L1 gene of bovine papillomavirus (BPV) in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.关键词
牛乳头状瘤病毒/L1基因/生物信息学分析/蛋白结构预测Key words
bovine papillomavirus (BPV)/L1 gene/bioinformatics analysis/protein structure prediction分类
生物科学引用本文复制引用
张海,周碧君,杨忠成,廖梅,王开功,文明,程振涛,王伟,冯旭芳..牛乳头状瘤病毒贵州株L1基因克隆及生物信息学分析[J].中国畜牧兽医,2016,43(11):2835-2843,9.基金项目
贵州省农业委员会科技专项《动物疫病防控能力提升专项》(黔农财[2015]149号) （黔农财[2015]149号）
"贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队"(黔科合人才团队[2015]4016号) （黔科合人才团队[2015]4016号）
贵州省黔南州社会发展科技重大计划项目《喀斯特石漠化林草牧综合治理技术研究与示范》[黔南社发科(20140325)] （20140325）
贵州现代肉牛产业技术体系专项(GZCYTX2015-0302-02) （GZCYTX2015-0302-02）
