中国畜牧兽医2016，Vol.43Issue(11)：2852-2858,7.DOI:10.16431/j.cnki.1671-7236.2016.11.007
猪繁殖与呼吸综合征病毒强、弱毒株Nsp9基因的反向遗传学替换改造
Substitution between Virulent and Avirulent PPRSV Vaccine Strains with Reverse Genetics System
摘要
Abstract
The Nsp9 gene of porcine reproductive and respiratory syndrome virus (PRRSV) coded the RNA polymerase,which played an important role in the virus replication.In order to determine the interaction between the Nsp9 gene and virulence,replication capacity and cell tropism,the Nsp9 gene sequence of PPRSV vaccine strain CH-1R was cloned,and ligated to a virulent strain XH-GD infectious clone after restriction enzyme digestion,and transformed the virus under the basis of the reverse genetics.The results showed that the virus was successfully saved.And the results of biological characteristics test showed that titers and growth rate of saved virus were obviously higher than that of parental strain rXH-GD,but were lower than vaccine strain CH-1R.While plaques of two strains were similar in size.The polymerase of vaccine strain CH-1R could help to improve the drop degree of the virus,and this test could lay the foundation for further study of the effect of Nsp9 gene on virus virulence.关键词
猪繁殖与呼吸综合征病毒/Nsp9基因/反向遗传学/病毒拯救Key words
PRRSV/Nsp9 gene/reverse genetics/virus salvation分类
生物科学引用本文复制引用
赵孟孟,钱娟娟,崔甜甜,冯松林,王文佳,邢星,冯嘉萍,张桂红..猪繁殖与呼吸综合征病毒强、弱毒株Nsp9基因的反向遗传学替换改造[J].中国畜牧兽医,2016,43(11):2852-2858,7.基金项目
国家自然科学基金:猪繁殖与呼吸综合征病毒非结构蛋白Nsp9功能研究项目(31272564) （31272564）
公益性行业(农业)科研专项经费(201203039) （农业）
国家生猪现代农业产业技术体系项目(CARS-36) （CARS-36）
国家重点研发计划课题(2016YFD0500707) （2016YFD0500707）
