中国畜牧兽医2016，Vol.43Issue(11)：2859-2865,7.DOI:10.16431/j.cnki.1671-7236.2016.11.008
VP22与EGFP、3种弓形虫抗原融合表达重组质粒的构建与鉴定
Construction and Identification of Cell Penetrating Peptides VP22 Gene Fused with Enhanced Green Fluorescent Protein and Three Antigens of Toxoplasma gondii
摘要
Abstract
To study a novel vaccine of Toxoplasma gondii,the recombinant plasmids that cell penetrating peptides VP22 gene fused with one enhanced green fluorescent protein (EGFP)(pcDNA-VP22-EGFP)and three antigens of T.gondii (pcDNA-VP22-SAG1,pcDNA-VP22-GRA4 and pcDNA-VP22-AMA1) were constructed,respectively.The recombinant plasmids were identified by PCR,restriction endonuclease digestion and DNA sequencing.The pcDNA-VP22-EGFP plasmid was transfected into COS7 cells,and the expression of VP22-EGFP in COS7 cells was confirmed by fluorescence microscope and RT-PCR.The results showed that all recombinant plasmid were constructed correctly.Green fluorescence was observed successfully under the fluorescence microscope in transfected cells after 72 h.The gene fragment of 1 449 bp was obtained by RT-PCR.The results indicated that the recombinant plasmids were constructed successfully.关键词
细胞渗透肽VP22/弓形虫/真核表达Key words
cell penetrating peptides VP22/Toxoplasma gondii/eukaryotic expression分类
农业科技引用本文复制引用
王娜,刘彦双,刘宁,金超,张喜路,金月,薛书江..VP22与EGFP、3种弓形虫抗原融合表达重组质粒的构建与鉴定[J].中国畜牧兽医,2016,43(11):2859-2865,7.基金项目
国家自然基金(31460657) （31460657）
吉林省科技厅青年科研基金(20150520129JH) （20150520129JH）
