中国畜牧兽医2016，Vol.43Issue(11)：3037-3046,10.DOI:10.16431/j.cnki.1671-7236.2016.11.034
不同佐剂对猪圆环病毒衣壳蛋白免疫原性的影响
Effects of Different Adjuvants on the Immunogenicity of PCV2-Cap Protein
摘要
Abstract
The study was aimed to prepare vaccines with different adjuvants,and research its effects on immunogenicity.The PCV2 Cap gene with its signal peptide removed was connected to pET-28a vector,and then was induced to express,using sodium deoxycholate (DOC) and low concentration of urea to dissolve the inclusion body.Different adjuvants,such as alum-based adjuvants,liposome adjuvants,propolis adjuvants,white oil adjuvants and Freund adjuvant were prepared,together with protein purified to make up subunit vaccines,and commercial inactivated vaccine as positive control,immuning mice,and ELISA method were used to detect changes in concentrations of animal serum antibody and cytokines,evaluated the immune protective effect.Results showed that the expression product in the form of inclusion body,using the DOC could omit dissolving inclusion body protein renaturation steps,and the purification method was simple,the capsid protein obtained had high purity.ELISA assays showed that PCV2-Cap had good immunogenicity.And we found that the water system adjuvants had high immune activity,this could provide important previous experimental data for the commercialization of the subunit vaccine.关键词
猪圆环病毒/包涵体/佐剂/亚单位疫苗Key words
porcine circovirus/inclusion body/adjuvant/subunit vaccine分类
生物科学引用本文复制引用
王立波,吴润生,闫超,吕暾..不同佐剂对猪圆环病毒衣壳蛋白免疫原性的影响[J].中国畜牧兽医,2016,43(11):3037-3046,10.基金项目
2013年福建省产学研重大专项"猪圆环病毒二代疫苗的开发"(2013N5006) （2013N5006）
2012年福州市产学研重大专项"猪圆环病毒二代疫苗的开发"(2012G106) （2012G106）
