中国畜牧兽医2016，Vol.43Issue(11)：3073-3079,7.DOI:10.16431/j.cnki.1671-7236.2016.11.039
一种新兽用止泻中药复方制剂对大鼠的急性及亚慢性毒性试验
Acute and Sub-chronic Toxicity Test on Rats of a New Kind of Antidiarrheal Chinese Herbal Medicine Compound Preparation for Livestock
摘要
Abstract
In order to understand the security of a new kind of antidiarrheal Chinese herbal medicine compound preparation for livestock,acute and sub-chronic toxicity test were conducted.Acute toxicity test used the largest drug dose method,20 Wistar rats were orally treated with the Chinese medicine compound preparation.In the sub-chronic toxicity test,80 rats were randomly divided into 4 groups with 20 rats in each group and orally given a dose of 3 000,1 500,750 and 0 mg/(kg·BW) of Chinese medicine compound preparation once a day for 30 days.The general clinical status was observed,rats weight were measured and the dose was adjusted every week during the test,after the test measured blood routine index,biochemistry index,and preceded the gross anatomy observation,weighing each major organs and calculated the viscera coefficient,and proceded main viscera histopathological observation between the high dose group and the control group.The acute toxicity results showed that every rat would be alive gavaged with the lethal dose (LD50) of compound preparation larger 5 g/(kg·BW).The sub-chronic toxicity autopsy showed that except heart,lung,and testicles in individual rats appeared mild bleeding in the high dose group,the other dose group organs found no abnormal change.The haematological index showed except mononuclear cell rate (P<0.05),and hematocrit declined significantly (P<0.05) in the high dose group,all the indexes of the other groups were in the normal range,there was no significant difference from the control group.The test suggested the Chinese medicine compound preparation was no toxicity under the condition of this test according to acute toxicity classification standard of exogenous chemicals by WTO,there was no effect on the growth and development of rats in the sub-chronic toxicity test,and there was no chronic toxicity at least 1 500 mg/kg feeding conditions in short-term repeated application.关键词
止泻/复方制剂/大鼠/急性毒性/亚慢性毒性Key words
antidiarrheal/compound preparation/rats/acute toxicity/sub-chronic toxicity分类
农业科技引用本文复制引用
姜玲玲,陈鸿宇,史开志,周思旋,余波,卜仕金..一种新兽用止泻中药复方制剂对大鼠的急性及亚慢性毒性试验[J].中国畜牧兽医,2016,43(11):3073-3079,7.基金项目
黔科合重大专项字[2013]6014 （）
黔科合LH字[2014]7701 （）
黔科合农G字[2015]4001 （）
黔科合NY[2015]3009-2 （）
