中国畜牧兽医2016，Vol.43Issue(12)：3085-3093,9.DOI:10.16431/j.cnki.1671-7236.2016.12.001
羊口疮病毒 GDZC 株锚蛋白基因的克隆及序列分析
Cloning and Sequence Analysis of Ankyrin Genes from Orf Virus GDZC Strain
摘要
Abstract
To investigate the role of ankyrin proteins encoding by Orf virus (ORFV)in the immune modulation to host infection,five ankyrin (ANK)genes of ORFV GDZC strain were amplified using specific primers by PCR amplification and cloning from the viral genome DNA which extracted from virus infection cells,and their sequences and coding protein structure bioinformatics analysis were performed. The results showed that ORFV008,ORFV123,ORFV126,ORFV128 and ORFV129 consisted of 516, 525,497,501 and 516 amino acids,respectively,which shared a nucleotide identity of 98.3%,98.7%, 97.9%,97.3% and 98.0% with those of ORFV OV-SA00 strain,respectively.All of five ANK pro-teins contained ANK and F-box structural domain that showed they were structure conservative proteins. The analysis of protein transmembrane showed that ORFV123 and ORFV128 had no potential trans-membrane domains,while the ORFV129,ORFV008 and ORFV126 had one,two and three potential transmembrane domains,respectively.And these ANK proteins had no signal peptide.These results provided the basic data for further study of the ANK proteins in the pathogenic and immune evasion mechanisms of ORFV.关键词
羊口疮病毒/锚蛋白/克隆/序列分析Key words
Orf virus/ANK protein/cloning/sequence analysis分类
农业科技引用本文复制引用
葛士坤,张凯照,刘健新,王晴楠,于梦,陶攀,宁章勇..羊口疮病毒 GDZC 株锚蛋白基因的克隆及序列分析[J].中国畜牧兽医,2016,43(12):3085-3093,9.基金项目
广东省科技计划项目(2016A020210079) （2016A020210079）
