中国畜牧兽医2016，Vol.43Issue(12)：3094-3100,7.DOI:10.16431/j.cnki.1671-7236.2016.12.002
Wip1过表达转基因小鼠模型的制备与鉴定
Establishment and Identification of Wip1 Over-expressed Transgene Mice Model
摘要
Abstract
This study was aimed to build Wip1 over-expressed transgene mice,which could pro-vide an animal model for clarifying the pathogenetic mechanism of atherosclerosis,and the trans-gene mice were screened at RNA level.Mice tissues cDNA were obtained by reverse transcription polymerase chain reaction (RT-PCR),which were used for the next PCR amplification template. The amplified fragments were used for gel recovery.Then,the amplified fragment and empty vec-tor were double enzyme digested.Full-length gene of Wip1,which was obtained by gel recovery, was connected to pcDNA3.1 (+)vector,and the Wip1-pcDNA3.1 (+)over-expressed vector was constructed.Finally,Wip1 over-expressed transgene mice were obtained.The transgene mice were identified and screened by Southern blotting and qPCR.30 Wip1 over-expressed trans-gene mice were got in this experiment,and the positive mice identified were 1 1 by Southern blot-ting,the positive rate was 36.67%.Moreover,the positive rate was 32.84% through screening by qPCR.Therefore,the Wip1-pcDNA3.1 (+)over-expressed vector was constructed success-fully,and the Wip1 over-expressed transgene mice were obtained through identifying and screen-ing at DNA and RNA level of Wip1 in mice tissues.关键词
Wip1/过表达/小鼠模型Key words
Wip1/over-expression/mouse model分类
生物科学引用本文复制引用
高倩,胡言青,唐懿挺,牟玉莲..Wip1过表达转基因小鼠模型的制备与鉴定[J].中国畜牧兽医,2016,43(12):3094-3100,7.基金项目
国家自然科学基金(31572378) （31572378）
