中国畜牧兽医2016，Vol.43Issue(12)：3114-3120,7.DOI:10.16431/j.cnki.1671-7236.2016.12.005
Nsp 9基因在 Marc-145细胞上对猪繁殖与呼吸综合征病毒复制的影响
Effect of Nsp9 Gene on Replication of PRRSV in Marc-145 Cells
摘要
Abstract
In order to evaluate whether Nsp9 could enhance the replication of porcine reproductive and respiratory syndrome virus (PRRSV),pIRES2-EGFP-Nsp9 plasmid containing whole Nsp9 genome were transfected into Marc-145 cells,Real-time PCR and Western blotting were used to evaluate the expression of N protein after PRRSV was inoculated.The results showed that the level of N protein in the Nsp9 transfected cells was significantly higher than control group (P <0.05),which was 1.5 times higher.Meanwhile,the expression at protein level had the same trend as the mRNA level.When the dose of plasmid was increasing,the expression of N protein both at the mRNA and protein levels were increased.In conclusion,Nsp 9 gene could enhance the replica-tion of PRRSV in Marc-145 cells.关键词
猪繁殖与呼吸综合征病毒/Nsp 9 基因/Marc-145 细胞/复制Key words
PRRSV/Nsp 9 gene/Marc-145 cell/replication分类
生物科学引用本文复制引用
赵孟孟,冯松林,王文佳,邢星,冯嘉萍,张桂红..Nsp 9基因在 Marc-145细胞上对猪繁殖与呼吸综合征病毒复制的影响[J].中国畜牧兽医,2016,43(12):3114-3120,7.基金项目
国家自然科学基金:猪繁殖与呼吸综合征病毒非结构蛋白 Nsp9功能研究(31272564) （31272564）
公益性行业(农业)科研专项经费(201203039) （农业）
国家生猪现代农业产业技术体系项目(CARS-36) （CARS-36）
国家重点研发计划课题(2016YFD0500707) （2016YFD0500707）
