中国畜牧兽医2016，Vol.43Issue(12)：3127-3134,8.DOI:10.16431/j.cnki.1671-7236.2016.12.007
猪圆环病毒2型山西株全基因组序列分析
Genome Sequence Analysis of Porcine Circovirus Type 2 Strains from Shanxi Province
摘要
Abstract
In order to study genetic variation of porcine circovirus type 2 (PCV2)strains in Shanxi province,the genomic sequences of four PCV2 strains which were SXXZ1,SXXZ2,SXTY and SXJZ were isolated recently from some areas of Shanxi province,they were amplified,cloned and sequenced.The amplified PCV2 genomic sequences of these four strains were analyzed and com-pared with that of 34 published PCV2 stains by DNAStar and drawing phylogenetic tree.The re-sults showed that the genomic sequences of SXJZ PCV2 strain was 1 768 bp,and the others were 1 767 bp,which accounted for 25% and 75%,respectively.The homologies of nucleotide se-quences of the four strains were 95.9% to 100.0%,the homologies of nucleotide sequences of the four strains with the 34 isolates from different regions of the world PCV strain were 94.5% to 99.9%,and the homologies of nucleotide sequences of the four strains with the domestic vaccine strains were 95.6% to 99.8%.The phylogenetic tree analysis showed that SXXZ1,SXXZ2 and SXTY belonged to PCV-2b genotype,and SXJZ belonged to PCV-2e genotype.The evolutionary rela- <br> tionship among SXXZ1,SXXZ2 and the QY strain of Guangdong were closer,we also found a recent evolutionary relationship between SXTY and AUT5 of Austria,and a recent evolutionary relationship between SXJZ and FJ of Fujian.Besides the evolutionary relationship among SXXZ1, SXXZ2,SXTY and the DBN-SX07-2 of PCV2 vaccine of Shanxi province,a recent evolutionary relationship between SXJZ and LG of PCV2 vaccine were found.Thus PCV2 strains were popular based on PCV-2b in Shanxi province,also PCV-2e was isolated and existed.It laid a foundation for the molecular epidemiology,genetic variation,prevention and control of PCV2 in Shanxi province.关键词
猪圆环病毒 2 型/全基因组/序列分析Key words
porcine circovirus type 2/genome/sequence analysis分类
农业科技引用本文复制引用
孟帆,李玉斌,吴忻,姚敬明,樊振华,刘文俊,王娟萍,韩一超,薛翼鹏,米瑞娟..猪圆环病毒2型山西株全基因组序列分析[J].中国畜牧兽医,2016,43(12):3127-3134,8.基金项目
山西省科技攻关项目(20130311026-4) （20130311026-4）
