中国畜牧兽医2016，Vol.43Issue(12)：3135-3140,6.DOI:10.16431/j.cnki.1671-7236.2016.12.008
鹿结核分枝杆菌 MPB70蛋白的表达与免疫原性鉴定
Expression of MPB70 Protein and Identification the Immunogenicity of Deer Mycobacterium tuberculosis
摘要
Abstract
In this study,the immunogenicity protein MPB 70 gene was amplified from Mycobacte-rium tuberculosis genome DNA which separated from deer,and about 590 bp fragment was ob-tained.Then the fragment was cloned and constructed prokaryotic expression vector of pET-30a-MPB70,and the recombinant plasmid was put into E .coli BL21 (DE3).Purified after IPTG in-duction,and analyzed by SDS-PAGE,a specificity protein band was observed at 20 ku.Using the deer serum positive of tuberculosis in Western blotting,the fusion protein could be combined with deer serum positive of tuberculosis antibody and arise specific immune response.The protein could be used as a specific antigen to test the deer tuberculosis.The study laid a foundation for further studying the deer tuberculosis appraisal method.关键词
结核分枝杆菌/MPB 70 基因/原核表达Key words
Mycobacterium tuberculosis/MPB 70 gene/prokaryotic expression分类
农业科技引用本文复制引用
王晓龙,卢天成,王秀然,杨艳玲..鹿结核分枝杆菌 MPB70蛋白的表达与免疫原性鉴定[J].中国畜牧兽医,2016,43(12):3135-3140,6.基金项目
吉林省科技攻关项目(20140204070NY) （20140204070NY）
