中国畜牧兽医2016，Vol.43Issue(12)：3170-3176,7.DOI:10.16431/j.cnki.1671-7236.2016.12.013
清瘟败毒散的质量标准提升研究
Research on the Promotion of Qingwenbaidu Powder Quality Standard
摘要
Abstract
To improve the quality standard of Qingwenbaidu powder,TLC was used to qualitative identity of Coptis ,Radix Rehmanniae ,Gardenia ,Forsythia ,Rhizoma Anemarrhena and Lo-phatherum gracile Brongn in Qingwenbaidu powder.The content of gardenin was determined by HPLC.Stationary phase were performed on ODS C18 column (4.6 mm ID×250 mm,5 μm)and the mobile phase was acetonitrile and water (13∶87),the detection wavelength was 238 nm.The results showed that in the relative position of the chromatography of the control medicinal materi-al,the chromatogram of the test sample showed the same color spots,and the negative sample had no interference.There was a good linear relationship (R2 =0.9999)when the garden in range of 0.062 to 0.370 μg.Its regression equation was y=1 789.1 x-14.296 and R2 =0.99984.The average recovery was 99.60% and RSD was 0.76%.This method was simple,accurate and re-producible,which could better control the quality of the Qingwenbaidu powder,and could be used as the quality standard of Qingwenbaidu powder.关键词
清瘟败毒散/质量标准/薄层色谱/测定Key words
Qingwenbaidu powder/quality standard/TLC/determination分类
农业科技引用本文复制引用
潘春晖,杨红玉,张煊,任园琴,彭晓凤,刘涛..清瘟败毒散的质量标准提升研究[J].中国畜牧兽医,2016,43(12):3170-3176,7.基金项目
四川省千人计划支持项目(川特聘第332号) （川特聘第332号）
