中国畜牧兽医2016，Vol.43Issue(12)：3306-3313,8.DOI:10.16431/j.cnki.1671-7236.2016.12.033
猪A群轮状病毒BJ株的分离与鉴定
Isolation and Identification of Porcine Group A Rotavirus BJ Strain
摘要
Abstract
Rotavirus infections are a major cause of viral diarrheas in young animals and children. Isolation and identification of rotavirus make a contribution to epidemiological survey and molecu-lar biology study.A strain of porcine rotavirus was isolated in MA104 cell cultures from the intes-tinal contents of piglets with diarrhea in Beijing.The virus was identified to be porcine rotavirus by immunochromatography strip test,Real-time RT-PCR,immunofluorescence test and sequen-cing analysis.According to the sequence analysis,the virus was classified as group A porcine ro-tavirus,the genotype ofVP 4,VP 6 and VP 7 genes belonged to P[13],I5 and G1 1,respectively. The virus was designated Rotavirus A pig/China/BJ/2015/G1 1P[13].关键词
猪轮状病毒/分离/鉴定Key words
porcine rotavirus/isolation/identification分类
农业科技引用本文复制引用
原霖,韩焘,倪建强,亢文华,汪葆玥,李晓霞,陈亚娜,翟新验,沈建忠..猪A群轮状病毒BJ株的分离与鉴定[J].中国畜牧兽医,2016,43(12):3306-3313,8.基金项目
科技部科技基础性工作专项---动物及动物产品质量安全检测标准物质研制(2013FY113300-6) （2013FY113300-6）
