中国畜牧兽医2016，Vol.43Issue(12)：3322-3328,7.DOI:10.16431/j.cnki.1671-7236.2016.12.035
上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析
Isolation,Identification and Analysis of Serotype and Drug Resistance of Salmonella from Pigeon in Shanghai
摘要
Abstract
The purpose of this study was to investigate the molecular types and antimicrobial re-sistance of Salmonella isolated from pigeon in Shanghai from 201 1 to 2014.A total of 92 fecal samples were collected from markets.XLD plate and Salmonella chromogenic medium were used to isolate suspected Salmonella colonies,and then determined the number of Salmonella through gram staining and biochemical tests.Among them,24 (26.1%)were positive for Salmonella . Kauffmann-White method and the K-B method were used respectively for serotype identification and susceptibility testing.In result,serological identification showed that 24 isolates from pigeon could be divided into 3 serotypes including S .typhimurium (66.7%),S .agona (25.0%)and S .corvallis (8.3%).Drug susceptibility test showed that 75.0% of the isolates were resistant to one antibiotic at least.The highest level resistance were found for tetracycline as well as sulfisox- <br> azole (62.5%),followed by streptomycin (58.3%),nalidixic acid (50.0%)and ampicillin (20.8%).Multi-drug resistant strains accounted for 62.5% (15),in which the largest number of strains (7,1 6.7%)were resistant to four drugs.In addition,isolates were 100.0% susceptible to cefepime,cefotaxime,ceftazidime,imipenem and ofloxacin,but 12.5% were moderately sen-sitive to the amoxicillin/clavulanic acid and ciprofloxacin.The study showed that Salmonella had a high separation rate of pigeon in Shanghai farmers market and performed serious multidrug re-sistance,which would bring great challenges and risks to the prevention and control of Salmonel-la in food,so that the prevalence and drug resistance of Salmonella in pigeon deserved close at-tention.关键词
肉鸽/沙门氏菌/血清型/耐药性Key words
pigeon/Salmonella/serotype/drug resistance分类
农业科技引用本文复制引用
梁德媚,许学斌,王伟芳,詹泽强,任行星,林嘉特,廖明,张建民..上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析[J].中国畜牧兽医,2016,43(12):3322-3328,7.基金项目
公益性行业(农业)科研专项(201403054) （农业）
广东省大学生科技创新培育专项资金(pdjh2016a0072) （pdjh2016a0072）
