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禽致病性大肠杆菌的分离、O 血清群鉴定及药物敏感试验

马兴树 朱美霞 石虎 杨广平

中国畜牧兽医2016,Vol.43Issue(12):3343-3348,6.
中国畜牧兽医2016,Vol.43Issue(12):3343-3348,6.DOI:10.16431/j.cnki.1671-7236.2016.12.038

禽致病性大肠杆菌的分离、O 血清群鉴定及药物敏感试验

Isolation,O Serogrouping and Drug Sensitivity Test of Avian Pathogenic Escherichia coli

马兴树 1朱美霞 1石虎 2杨广平2

作者信息

  • 1. 河北工程大学农学院分子生物学实验室,邯郸 056021
  • 2. 河北科星药业有限公司河欣科技中心,石家庄 050200
  • 折叠

摘要

Abstract

One hundred and ninety four avian pathogenic Escherichia .coli (APEC)isolated from different pathotypes of chickens in Hebei province,Shandong province,Henan province,Jiangsu province,Shanxi province and Liaoning province in China between May 2005 and March 201 6 were investigated by determination of biochemical characters,O serogroup identification and drug sensi-tivity test.Out of 1 94 isolates,124 strains(63.9%)could be grouped to serogroups O1 (3),O2 (26),O57(15),O65(47)and O78(33).The drug sensitivity test used eight antibiotics (ampicillin, neomycin,doxycycline,sulfamonomethoxine,tilmicosin,ciprofloxacin ceftiofur and florfenicol) by one strain of O1,O2,O57,O65,O78 serogroup,respectively.The results showed that all strains were sensitive to ciprofloxacin and neomycin,and resistance to other drugs at varying de-grees.This study demonstrated that the high prevalence of O65,O78,O2 and O57 isolates existed and provided the basis for the development of multivalent bacterin in future.

关键词

禽致病性大肠杆菌/分离/鉴定/O 血清群/药物敏感试验

Key words

avian pathogenic E .coli/isolation/identification/O serogroups/drug sensitivity test

分类

农业科技

引用本文复制引用

马兴树,朱美霞,石虎,杨广平..禽致病性大肠杆菌的分离、O 血清群鉴定及药物敏感试验[J].中国畜牧兽医,2016,43(12):3343-3348,6.

基金项目

河北省科技厅科技支撑计划项目(13226602D) (13226602D)

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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