中国畜牧兽医2016，Vol.43Issue(12)：3356-3362,7.DOI:10.16431/j.cnki.1671-7236.2016.12.040
奶牛乳房炎白色念珠菌的分离鉴定及生物学特性研究
Isolation,Identification and Analysis of Biological Characteristics of Candida albicans in Bouine Mastitis
摘要
Abstract
The study was aimed to learn the infection of dairy cows with fungi in Ningxia and take effective measures to prevent and control the fungi infection.Milk samples were collected from dairy cows that did not respond to antibiotics in Ningxia,fungi in milk samples were separated and identified,and the biological characteristics of isolated bacteria were analyzed.Milk samples infected by fungus were screened out using fungal selective medium.The yeast pathogen was shown to be Candida albicans by biochemistry and molecular biology methods.Determination of enzyme activity and biofilm-forming tests confirmed that it had different levels of virulence with strong pathogenicity to experimental animals.Drug sensitive test was carried out by K-B pro-grame,the results showed that the isolates were resistant to clinical commonly used antibiotics, and were sensitive to clinical common antifungal drugs.The fungal mastitis was caused by Can-dida albicans in Ningxia,the ratio of fungal infection was 43%.Candida albicans had strong vir-ulence and were resistant to common antibiotic and sensitivity to antifungal drugs and traditional Chinese medicine.关键词
白色念珠菌/乳房炎/致病性/溶血性Key words
Candida albicans/mastitis/pathogenicity/haemolysin分类
农业科技引用本文复制引用
何芳,卢金霞,冯峰,周学章..奶牛乳房炎白色念珠菌的分离鉴定及生物学特性研究[J].中国畜牧兽医,2016,43(12):3356-3362,7.基金项目
国家自然科学基金项目(31660728) （31660728）
