中国卒中杂志2016，Vol.11Issue(12)：1035-1039,5.DOI:10.3969/j.issn.1673-5765.2016.12.008
姜黄素对大脑中动脉缺血模型大鼠的神经保护作用及机制研究
Curcumin Protects Rat Brains Against Cerebral Ischemia by Down-regulating the Expression of Notch 1 and NF-κB
李姝雅 1王伊龙 1王春雪 1刘改芬 1王拥军1
作者信息
- 1. 100050 北京首都医科大学附属北京天坛医院神经内科
- 折叠
摘要
Abstract
Objective To investigate the relationship between CUR and the expression of Notch 1 and NF-κB, and evaluate the neuroprotection of CUR in rat permanent middle cerebral artery occlusion (MCAO). Methods A total of ninety-three male, Sprague-Dawley rats were subjected to MCAO. Among which, sixty-three rats were used to investigate the relationship between CUR and the expression of Notch 1 and NF-κB after cerebral ischemia, and 30 rats were used to detect the effect of CUR in the acute phase of ischemic stroke. Neurological deifcit, brain water content and infarct sizes were measured at 48 h after MCAO. Immunohistochemistry and Western blot were used to analyze the expression of Notch 1 and NF-κB. Results Compared with sham group, the expression of Notch 1 was up-regulated at early stage after cerebral ischemia. CUR down-regulated the expression of Notch 1 and NF-κB, and the inhibition effect lasted at least 72 hours after MCAO (P<0.05). Compared with vehicle-control group, CUR dramatically improved neurological deifcit at 48 h (P<0.05), reduced brain water content [(80.42±9.00)%vs (83.71±7.00)% (P<0.05)] and infarct volume [(40.08±3.66)%vs (28.94±6.20)%(P<0.05)].Conclusion CUR protects the brain from damage caused by cerebral ischemia; this effect may be through down-regulating the expression of Notch 1 and NF-κB.关键词
缺血性脑血管病/姜黄素/炎症反应/Notch 1/NF-κBKey words
Cerebral ischemia/Curcumin/Inlfammation/Notch 1/NF-κB引用本文复制引用
李姝雅,王伊龙,王春雪,刘改芬,王拥军..姜黄素对大脑中动脉缺血模型大鼠的神经保护作用及机制研究[J].中国卒中杂志,2016,11(12):1035-1039,5.
