浙江大学学报(理学版)2016,Vol.43Issue(6):751-755,5.DOI:10.3785/j.issn.1008-9497.2016.06.022
铬胁迫对西红花叶片蛋白表达谱的影响
Effect of chromium stress on protein profiles in saffron
摘要
Abstract
Chromium (Cr) is a nonessential element for plants .Cr not only affects a series of physiological processes in plants ,but also significantly inhibits the grow th and development of plants .Under Cr stress ,plants have evolved complex detoxification mechanisms .In the present study ,we investigated the protein expression of leaves of saffron in response to Cr stress via 2D electrophoresis and mass spectrum analysis .Nine proteins were identified to be down-regulated under Cr stress ,including cell division cycle protein 48 homolog ,ATP synthase subunit alpha ,ribu-lose bisphosphate carboxylase large chain ,Proteasome subunit alpha type ,etc .Six proteins were up-regulated ,in-cluding sucrose synthase 2 ,eukaryotic initiation factor 4A ,Alpha-1 ,4-glucan-protein synthase ,1-aminocyclopro-pane-1-carboxylate oxidase and Isoflavone reductase homolog IRL ,etc .The results provide valuable insights to-wards the molecular mechanism of saffron in response to Cr toxicity .关键词
铬/西红花/叶片/蛋白组Key words
chromium/saffron/leaf/proteomics分类
医药卫生引用本文复制引用
饶君凤,吕伟德,曹方彬..铬胁迫对西红花叶片蛋白表达谱的影响[J].浙江大学学报(理学版),2016,43(6):751-755,5.基金项目
科技部国家星火计划项目(2015GA700053) (2015GA700053)
浙江省自然科学基金资助项目(LY13B020001) (LY13B020001)
浙江省科技厅公益技术研究农业项目(2015C32104) . (2015C32104)