北京中医药大学学报2016，Vol.39Issue(12)：1022-1026,5.DOI:10.3969/j.issn.1006-2157.2016.12.010
益精方对腺嘌呤不育症大鼠睾丸支持细胞间连接黏附分子和闭合小环蛋白表达的作用
Effect of Yijing Fang on expression of junctional adhesion molecules and zonula occludens in testicular sertoli cells of adenine-induced infertile rats
摘要
Abstract
Objective To observe the effect of Yijing Fang,a TCM compound,on the expression of junctional adhesion molecules (JSM)and zonula occludens (ZO)in testicular sertoli cells of infertile rats with kidney-yang deficiency pattern induced by adenine.Methods Altogether 48 healthy male Wistar rats of SPF grade,two-month-old,were divided according to random number table,into blank group, model group,Fufang Xuanju group and Yijing Fang group(n =12 each).After modeling,the rats were intragastrically administered by corresponding medicines for consecutive 20 days.Then the rats were sac-rificed and their testis and epididymis were resected.The sperm density and sperm activity were calcula-ted using Computer Assisted Sperm Analysis System,and the expression of JAMand ZO in testicular ser-toli cells were measured using immunohistochemical method.Results Compared with the normal group, the sperm density,sperm activity rate and expression of JAMand ZO declined significantly(P <0.01). Compared with the model group,the sperm density and the sperm activity rate increased in Fufang Xuan-Ju group and Yijing Fang group remarkably (P <0.05),while expression of JAM and ZO increased in Yijing Fang group((P <0.05 ).Conclusion Yijing Fang can repair the damage of testicular sertoli cells of kidney-yang deficiency pattern rats induce by adenine,via regulating the expression of JAM and ZO.关键词
益精方/腺嘌呤/不育/肾阳虚/连接黏附分子/闭合小环蛋白/大鼠Key words
Yijing Fang/adenine/infertile/pattern of kidney-yang deficiency/junctional adhesion mole-cules/zonula occludens/rats分类
医药卫生引用本文复制引用
郑小挺,陈东,尹申,马玲,王家辉..益精方对腺嘌呤不育症大鼠睾丸支持细胞间连接黏附分子和闭合小环蛋白表达的作用[J].北京中医药大学学报,2016,39(12):1022-1026,5.基金项目
国家自然科学基金项目 （）
