北京中医药大学学报2016，Vol.39Issue(12)：1041-1047,7.DOI:10.3969/j.issn.1006-2157.2016.12.001
不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响
Influence of electro-acupuncture of different frequency and stimulation times on skin blood perfusion around Neiguan (PC6)and sham acupoint in rats with myocardial ischemia
摘要
Abstract
Objective To compare the skin blood perfusion around Neiguan (PC 6)and sham acupoint needled by the different frequency and stimulation times of electro-acupuncture (EA),in healthy rats and myocardial ischemic rats.Methods According to random number table,altogether 100 male Wistar rats were divided into once treatment group and three times treatment group,which were both divided into control group,sham-operation group,model group,EA low-frequency group and EA high-frequency group (each n =10).After successful modeling,rats of EA groups were acupunctured at left Neiguan (PC 6)acupoint.60 minutes after last treatment of acupuncture,skin blood perfusion around bilateral Neiguan(PC 6)acapoints and sham acupoint were tested by laser speckle contrast imaging technology (LSCI) and analyzed,and the myocardial morphological changes were observed by HE staining. Results Compared with control group,the skin blood perfusion around PC 6 in model groups decreased significantly after three time treatment (P <0.01 ),and that around PC 6 or sham acupoint in model group of three times treatment group was much less than that of once treatment group (P <0.01).Com-pared with the model group,the skin blood perfusion around PC 6 both significantly increased after low-frequency EA or high-frequency EA after three times treatment (P <0.01).Conclusion The variation of skin blood perfusion around PC 6 should be useful for specific reflection of normal condition or ische-mic condition of rats myocardium,but that around sham acupoint didn’t.The effect of three-times-treat-ment against declination of skin blood perfusion due to myocardial ischemia was superior to that of once-treatment regardless of high frequency or low frequency,and EA of high frequency and three-times stimu-lation showed the significant effect.However,the mechanism remains to be further studied.关键词
心肌缺血/电针/内关/非经非穴/皮肤血流灌注量/经穴脏腑相关/大鼠Key words
myocardial ischemia/electro-acupuncture/Neiguan(PC 6)/sham acupoint/skin blood per-fusion/correlated acupoints and viscera/rats分类
医药卫生引用本文复制引用
路雅雯,毛颖秋,李志刚,程凯,嵇波,戴健,赵国桢,王丹,严明娜,白红新,孙晓敏,郭孟玮..不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响[J].北京中医药大学学报,2016,39(12):1041-1047,7.基金项目
国家自然科学基金面上项目（No．81373558），北京中医药大学自主选题项目 （）
