重庆医学2016，Vol.45Issue(36)：5056-5058,3.DOI:10.3969/j.issn.1671-8348.2016.36.005
三脉紫菀有效组分配比的平喘作用及其毒性研究
Study on the effective compositions of Aster ageratoides Turcz for relieving asthma and its toxicity
摘要
Abstract
Objective To study the effective composition of Aster ageratoides Turcz for relieving asthma and its toxicity . Methods Seventy‐two qualified male guinea pigs ,body mass(300 ± 20)g ,were randomly divided into the control group ,aminophyl‐line group(0 .047 g/kg) and different ratio group of total saponins and total flavonoids (8∶1 ,4∶1 ,2∶1 ,1∶1 ,1∶2 ,1∶4 ,1∶8) . Every group received at dose of 0 .38 g/kg once a day for 3 days by intragastric administration .The effect of relieving asthma was taken as the indicators for the optimization of compositions ,which was detected by observing the latent period of asthma induced by acetylcholine‐histamine in guinea rats .The relaxation percentage of smooth muscle retracted by acetylcholine was detected by Pow‐erlab biological signal collecting system when aminophylline(1 .25 mg/mL) and different matching drugs(0 .02 g/mL) were added in Krebs′solution .The acute toxicities of total saponins ,total flavonoids and mixture of total saponins with total flavonoids (ratio of 1∶2) were detected by classical acute toxicity test of mice .Results Compared with the control group ,aminophylline group ,each group latent period of inducing asthma significantly or very significantly prolonged (P<0 .05) ,among them ,1∶1 ,1∶2 ,1∶4 group and other matching group were significantly different(P<0 .05) ,but 1∶2 group cited the longest incubation period .Aminophylline group and the ratio could significantly relaxe guinea pig tracheal smooth muscle retracted by acetylcholine .Aminophylline group di‐astolic percentage was the largest(24 .12 ± 1 .20)% ,all the groups were less than the percentage of diastolic aminophylline group (P<0 .05) ,but in 1∶2 group with the closest .Aster ageratoides Turcz ,tatarian aster total saponins ,total flavonoids and the ratio of 1∶2 to mice maximum tolerance was respectively 300 ,345 ,325 g/kg .Conclusion For the same total dosage ,the relieving asth‐ma effects of proportions of 1∶2 Aster ageratoides Turcz were obvious .There are no abnormal changes in the acute toxicity trila with total flavonoids ,total saponins ,as well as the ratio of 1∶2 ,safety is relatively good .关键词
三脉紫菀/皂苷类/黄酮类/平喘/急性毒性Key words
Aster ageratoides Turcz/saponins/flavone/antiasthma/acute toxicity分类
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康林之,赵海平,朱建成,任刚,罗云,张婧,刘荣华,杨明,王芳,何小汝..三脉紫菀有效组分配比的平喘作用及其毒性研究[J].重庆医学,2016,45(36):5056-5058,3.基金项目
十一五重大新药创制资助项目（2009ZX09310－005）；江西省科技厅资助项目（20111BBG70005－4）；江西中医药大学校基金资助项目（Y027）。 （）
