重庆医学2016，Vol.45Issue(36)：5069-5071,3.DOI:10.3969/j.issn.1671-8348.2016.36.009
基于MEBT／MEBO的ERK1／2和p38信号通路与糖尿病足免疫表达关系的研究
The relationship between ERK1/2 and p38 signaling pathway with the immune expression of diabetic based on MEBT/MEBO
摘要
Abstract
Objective To investigate the regulation on extracellular signal regulated kinase (ERK )1/2 and p38 signaling pathways and immune expression by moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)in diabetic foot and their relationship ,to explore the repair mechanism of MEBT/MEBO on diabetic foot ulcers .Methods Totally 40 diabetic foot pa‐tients were treated by MEBT/MEBO ,to take wound tissue before and after treatment and detect the expression of ERK 1/2 ,p38 , MAPKK6 ,c‐myc ,Akt ,ATF2 ,IgA ,IgM ,IgG ,C3c and C4c by immunohistochemistry ,to investigate their relationship .Results Af‐ter treatment with MEBT/MEBO ,the area of foot wounds in 39 patients was reduced in different degree .Only one patient had no obvious change .14 patients(35 .00% ) were markedly effective ,25 patients(62 .50% ) and 1 patient(2 .50% ) were ineffective .Before andaftertreatment,allpositiveexpression,positiveimmunoreactivity(anyindex)andpositiveexpression(specificindex)ofsignal pathway molecules were statistically significant (P<0 .05) .While the positive rate of molecular expression in wound pathway in‐creased ,the positive expression rate of immune factor increased .Before treatment ,a small amount of immune factors were found in the wound tissue .After treatment ,the immune factors IgA ,IgM ,IgG ,C3c ,C4c were distributed widely and diffusely .Before treat‐ment ,the wound tissue showed a very small number of signal molecules .After treatment ,the signal pathway molecules MEBT/MEBO and p38 ,MAPKK6 ,c‐myc ,Akt ,ATF2 showed broad and diffuse distribution .Conclusion MEBT/MEBO may promote the expression of ERK1/2 and p38 signaling molecules and immune in diabetic foot ,p38 and ERK1/2 signaling pathway may promote the healing of diabetic foot wound by increasing the expression of immune .关键词
糖尿病足/烧伤皮肤再生医疗技术/细胞外信号调节激酶1/2/p38/免疫Key words
diabetic foot/burn skin regenerative medical technique/extracellular signal regulated kinase1/2/p38/immune分类
医药卫生引用本文复制引用
吴标良,唐乾利,覃晓洁,冯烈,王民登,何明杰,吕震..基于MEBT／MEBO的ERK1／2和p38信号通路与糖尿病足免疫表达关系的研究[J].重庆医学,2016,45(36):5069-5071,3.基金项目
国家自然科学基金资助项目（81360547）；2013年广西自然科学基金重点项目（2013GXNSFDA019020）。 （）
