南方农业学报2016，Vol.47Issue(10)：1642-1647,6.DOI:10.3969/jissn.2095-1191.2016.10.1642
甘蔗与斑茅割手密复合体的回交后代真实性鉴定及染色体遗传分析
Authenticity identification and chromosomes genetic analysis of backcross progeny between sugarcane(Saccharum officinarum) and intergeneric hybrid complex(Erianthus arundinaceus × Saccharumspontaneum)
摘要
Abstract
Objective]Inheritance of chromosome was studied in the process of hybridization between sugarcane(Saccha-rum officinarum) and intergeneric hybrid complex(Erianthus arundinaceus × Saccharum spontaneum) to lay a good founda-tion for exploration of optimum backcross generations. [Method]The backcross progenies from sugarcane (S. officinarum) and intergeneric hybrid (E. arundinaceus×S. spontaneum) were identified with four pairs of SSR primers and chromo-some number for the hybrids was calculated by means of cell wall degradation hypotonic smear method. [Result]All 28 progenies, 15 BC1 progenies from GT05-164 and GXASF108-2-28 and 13 BC2 progenies from GT42 and GXASBC112-A6-25, were confirmed as hybrids. Chromosome numbers of progenies(five BC1 hybrids and seven BC2 hybrids) in which am-plification of paternal characteristics band occurred in two pairs of SSR primers were observed. The somatic chromosome num-bers of five BC1 hybrids and seven BC2 hybrids were 95-98 and 94-101, respectively, all following the law of n+n. [Conclu-sion]Chromosome transmission in BC1 and BC2 hybrids of backcross progeny from sugarcane and intergeneric hybrid follows n+n mode. Chromosome numbers female parent sugarcane(S. officinarum) are not doubled, and nobilisation progress slows down. More generations may be needed to obtain superior varieties(lines) of E. arundinaceus and S. spontaneum consanguinity.关键词
甘蔗/斑割复合体/杂交后代/SSR标记/染色体传递Key words
sugarcane/intergeneric hybrid complex(Erianthus arundinaceus×Saccharum spontaneum)/hybrids/SSR marker/chromosome transmission分类
农业科技引用本文复制引用
黄玉新,张革民,李杨瑞,罗霆,高轶静,张保青,周珊,杨翠芳,段维兴,雷敬超,韦金菊..甘蔗与斑茅割手密复合体的回交后代真实性鉴定及染色体遗传分析[J].南方农业学报,2016,47(10):1642-1647,6.基金项目
广西科技重点研究计划项目（桂科AB16380126）；广西自然科学基金项目（2014GXNSFBA118119）；广西农业科学院基本科研业务费专项项目（2015YT04）；广西八桂学者专项项目 （）
