南方农业学报2016，Vol.47Issue(10)：1698-1703,6.DOI:10.3969/jissn.2095-1191.2016.10.1698
广西甜瓜细菌性果斑病病原鉴定及16S rDNA序列分析
Identification of pathogen causing melon bacterial fruit blotch in Guangxi and analysis of its 16S rDNA sequence
摘要
Abstract
Objective]In order to provide a basis for disease-resistant breeding and integrated management of bacteri-al fruit blotch, suspected pathogen causing melon bacterial fruit blotch was isolated and identified. [Method]Suspected leaves and fruits samples were collected from Nanning(No.WM0922) and Hezhou(No.XD0611),and pathogen was isolated and purified from samples,then identified based on pathogenicity test,colony morphology and cultivate characteristics, physiological and biochemical characteristics and 16S rDNA sequence analysis . [Result]Results showed that , five germs were isolated from suspected leaves(No.WM0922),and three of which(No.WM0922-2,WM0922-3,WM0922-4) were pathogenic. Eight germs were isolated from suspected fruits (No.XD0611),four of which (No.XD0611-5,XD0611-6、XD0611-7,XD0611-8) were pathogenic. Two representative germs WM0922-3 and XD0611-7 were selected for follow-up test,and the test result showed that they could infect gourd leaves,and were identified as Gram-negative bacterium,with same culture characters,physiology and biochemistry,and their 16S rDNA sequences shared more than 99%similarily with that of Acidororax citrulli. [Conclusion]The pathogen causing melon bacterial fruit blotch is identified as A. avenae subsp. citrulli.关键词
甜瓜/果斑病菌/病原菌鉴定/燕麦嗜酸菌西瓜亚种Key words
melon/bacterial fruit blotch/pathogen identification/Acidororax avenae subsp. citrulli分类
农业科技引用本文复制引用
谢慧婷,李战彪,秦碧霞,杨世安,崔丽贤,邓铁军,蔡健和..广西甜瓜细菌性果斑病病原鉴定及16S rDNA序列分析[J].南方农业学报,2016,47(10):1698-1703,6.基金项目
国家现代农业产业技术体系广西特色水果创新团队项目（nycytxgxcxtd-04-19-2）；广西农业科学院科技发展基金项目 （）
