工业微生物2016，Vol.46Issue(6)：7-12,6.DOI:10.3969/j.issn.1001-6678.2016.06.002
微生物转化连翘苷制备连翘脂素的研究
Production of phillygenin from phillyrin by microbial bioconversion
梅建凤 1董志红 1易喻 1陈建澍 1张彦璐 1应国清1
作者信息
- 1. 浙江工业大学 药学院,浙江 杭州310014
- 折叠
摘要
Abstract
A bioconversion process was developed for production of phillygenin from its glycosidic form—phillyrin. A strain of Penicillium citrinum designated as LB was isolated from the soil, which could specifically produce enzyme for bioconversion of phillyrin to phillygenin. After main composition for LB strain producing enzyme and bioconversion conditions were optimized, a high molar yield of phillygenin could be obtained. The optimized medium contained sucrose 7 g/L, (NH4)2SO45 g/L, NaCl 5 g/L, KH2PO45 g/L, MgSO41 g/L, MnSO40. 5 g/L, with pH 6. 0. After the strain LB was cultivated in enzyme-producing medium for 2 days, the cells were harvested by filtration and suspended in pH 6. 0 phosphate buffer at the volume of 2 times of the culture. 2 g/L of phillyrin was added in the cell suspension, and the bioconversion was carried out at 30 ℃, 200 r/min for 20 h. The molar yield of phillygenin could reach 94. 1%. The developed process had advantages of simple operation, high productivity, convenience for product purification and less by-products, which might be a potential way for production of phillygenin in the pharmaceutical industry.关键词
连翘苷/连翘脂素/生物转化/桔青霉Key words
phillyrin/phillygenin/bioconversion/Penicillium citrinum引用本文复制引用
梅建凤,董志红,易喻,陈建澍,张彦璐,应国清..微生物转化连翘苷制备连翘脂素的研究[J].工业微生物,2016,46(6):7-12,6.
