工业微生物2016，Vol.46Issue(6)：24-33,10.DOI:10.3969/j.issn.1001-6678.2016.06.005
高产洛伐他汀棒曲霉菌株的筛选、鉴定及发酵条件优化
Screening and identification of Aspergillus clavatus strain with high-yielding lovastatin and its optimization of fermentation conditions
摘要
Abstract
150 Aspergillus strains were selected from different natural fermentation samples of food, soil, air and so on, and their products of lovastatin were measured by high performance liquid chromatography ( HPLC ) method. Among them, the Aspergillus strain Ac-32 producing higher-level lovastatin was screened. Based on 18S rDNA sequence and morphology characteristics, the strain Ac-32 was identified as Aspergillus clavatus. Through analyzing the influence of C source, N source, C content, N content, C/N, temperature, initial pH and so on, the results indicated that optimal fermentation conditions were as follows:C source lactose, N source peptone, C content 100 g/L, N content 12 g/L, C/N 15:1. 8, temperature 28 ℃, shaking speed 180 r/min, initial pH 5. 2, inoculating age 4 d and inoculum size 6%. Using Plackett-Burman ( P-B) in Minitab 17, 4 significant effects ( C content, N content, temperature and pH) on lovastatin yields were screened out. According to the P-B experiment results and analysis of response surface methodology in Design Expert, the optimum conditions were as follows:C content 100 g/L, N content 11. 8 g/L, temperature 28 ℃, pH 5. 2. Under the optimized conditions, the maximum lovastatin yield was 236. 221 μg/mL.关键词
棒曲霉/洛伐他汀/响应面/发酵条件Key words
Aspergillus clavatus/Lovastatin/response surface methodology/fermentation conditions引用本文复制引用
章婷,Bedelkhan Almagul,郑婕施,韩肖飞,任浩,蒋冬花..高产洛伐他汀棒曲霉菌株的筛选、鉴定及发酵条件优化[J].工业微生物,2016,46(6):24-33,10.基金项目
国家自然科学基金项目(NO. 31270061 （）
NO. 31570013). （）
