工业微生物2016，Vol.46Issue(6)：41-46,6.DOI:10.3969/j.issn.1001-6678.2016.06.007
共存碳源对克雷伯氏菌NIII2发酵蔗糖产絮凝剂的影响
Effects of co-carbon source compounds on production of bioflocculant by Klebsiella sp. NIII2 with sucrose
摘要
Abstract
Effects of co-carbon source compounds on the production of bioflocculant by Klebsiella sp. NIII2 with sucrose were investigated. The results showed that with citrate as co-carbon source compounds in fermentation medium, NIII2 was easy to produce acid, which often resulted in decrease of the bioflocculant yield and carbon conversion rate. With succinic acid, acetate or lactate as co-carbon source, the values of pH were higher than 7. 5, the yield could reach the highest of 10. 87 g/L and the carbon conversion rate was as high as 43. 48%. Compared with citrate as co-carbon source, succinate as co-carbon source could make the ratio of protein and saccharide increase by 33%; Zeta potential flocculation activity was improved from-60 . 00 mV to -28 . 07 mV;the particle size of bioflocculant widely distributed in the range of 0~300μm and the proportion of large particle size was augmented. With the increasing high degree polymerization of the bioflocculant and with flocculation surface rendering agglomerate amorphous structure, it improved the flocculation activity and performance. With a 4. 0 mg/L dose of such bioflocculant, the removal rate of the suspended solid in kaolin solution (2 g/L) was up to 97. 3%.关键词
共存碳源/克雷伯氏菌NIII2/微生物絮凝剂/碳源转化率Key words
co-carbon source/Klebsiella sp. NIII2/microbial flocculant/carbon conversion rate引用本文复制引用
白雪蕊,聂麦茜,谢铮胜,宋勃轩,聂红云..共存碳源对克雷伯氏菌NIII2发酵蔗糖产絮凝剂的影响[J].工业微生物,2016,46(6):41-46,6.基金项目
陕西省重点科技创新团队建设计划(编号:2013KCT-13) （编号:2013KCT-13）
国家自然科学基金(编号:51278405). （编号:51278405）
