摘要
Abstract
The fermentation conditions of NMN adenylyltransferase production by recombinant Escherichia coli were optimized. Biomass yield ( OD600 ) and NMN adenylyltransferase activity were selected as response value during fermentation condition optimization. Peptone, glycerin and MgSO4 were screened to be the three key components using Plackett-Burman design method. Then, the mathematical model reflecting the three main factors and the production level of enzyme was established by using the Box-Behnken design method. Finally, a satisfactory degree function was defined and the satisfactory solution was obtained. The final optimal fermentation conditions were:yeast extract 30 g/L, peptone 10. 5 g/L, glycerol 3. 49 mL/L, MgSO40. 45 g/L, K2HPO440. 5 g/L, KH2PO46. 0 g/L, NH4Cl 1. 5 g/L, NaCl 0. 6 g/L, inoculum size of 1. 5% and induction time of 12 h. After the validated experiment under above conditions, enzyme production and biomass of recombinant strain were increased by 74. 92% and 23. 92%, respectively.关键词
NMN转移酶/发酵优化/满意度函数/响应面法Key words
NMN adenylyltransferase/fermentation optimization/desirability function/response surface methodology
