解放军医药杂志2016，Vol.28Issue(12)：1-5,5.DOI:10.3969/j.issn.2095-140X.2016.12.001
去甲基脱氧鬼臼毒素衍生物DPPC诱导A549细胞凋亡的研究
A Study Demethyl -deoxypodophyllotoxin Derivative DPPC Induced A549 Cells Apoptosis
摘要
Abstract
Objective To investigate effect of podophyllotoxin derivative L-N-formycl-4'-demethyldeoxypodophyl-lotoxin-Methyl-4-N-Piperazine acidamide ( DPPC) in inhibition of proliferation of human non-small cell lung cancer A549 cells, and the molecular mechanism of DPPC induced apoptosis in A549 cells. Methods The A549 cells were cultured in vitro, and apoptoses were detected using Hochest33258 after A549 cells were treated with DPPC. Apoptosis number af-ter DPPC treatment was detected by double-dye quantitative detection of Annexin V-FITC/PI. Expressions of apoptosis genes P53, caspase-3 and Bax were detected by protein immunoblot test. The above values in DPPC group were compared with those in Etoposide ( positive control) group and A549 cells without any medication treatment group ( negative control group). Results γ-H2AX expression of A549 cells in DPPC group were significantly increased compared with those in negative and positive control groups. A549 cells after DPPC treatment were nucleus atrophy and cell apoptotic body under fluorescence microscope. Compared with those in negative control group, inhibited tumorigenesis and development p53 gene expressions of A549 cells after different concentrations of DPPC treatment were increased, and the expression showed dose dependent, and expressions of apoptosis related protein caspase-3 and Bax were increased in the downstream (P<0. 05, P<0. 01). The same concentration of DPPC and VP16 inhibited the apoptosis related proteins showed signif-icant difference (P<0. 05). Conclusion DPPC can cause DNA damage of A549 cells, induce apoptosis, make abnor-mal expressions of apoptosis related proteins in cells and inhibit proliferation of A549 cells. In addition, DPPC shows su-perior effect of anti-tumor cell proliferation in vitro.关键词
去甲基脱氧鬼臼毒素衍生物/癌,非小细胞肺/DNA损伤,细胞凋亡Key words
Demethyl-deoxypodophyllotoxin derivative/Carcinoma/non-small-cell lung/DNA damage/Apop-tosis分类
医药卫生引用本文复制引用
桑春艳,杨翠霞,丁洁芳,杨霄鹏,惠玲..去甲基脱氧鬼臼毒素衍生物DPPC诱导A549细胞凋亡的研究[J].解放军医药杂志,2016,28(12):1-5,5.基金项目
国家自然科学基金资助面上项目(81372177) （81372177）
全军医药卫生科研基金课题资助项目(CLZLZJB04) （CLZLZJB04）
